The Tag-lite® ligand binding assay is a homogeneous alternative to radioligand binding assays for HTS and compound profiling. The assay is based on the competition between the fluorescent ligand and compound.
Vasopressin V1A receptor ligand binding assay
Assay Validation
Please note that the data below should be considered only as an example. Results may vary from one HTRF compatible reader to another.
The Vasopressin V1A receptor ligand binding assay was performed using:
- cells expressing the SNAP-receptor labeled with terbium cryptate
- ligand labeled with a red-emitting HTRF fluorescent probe
Results obtained with this assay were very similar to those obtained with the radio ligand reference method.
Literature
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Title
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Time-resolved FRET between GPCR ligands reveals oligomers in native tissues.
Albizu L, Cottet M, Kralikova M, Stoev S, Seyer R, Brabet I, Roux T, Bazin H, Bourrier E, Lamarque L, Breton C, Rives ML, Newman A, Javitch J, Trinquet E, Manning M, Pin JP, Mouillac B, Durroux T.
Nat Chem Biol. 2010;6(8):587-94.
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Toward efficient drug screening by homogeneous assays based on the development of new fluorescent vasopressin and oxytocin receptor ligands.
Albizu L, Teppaz G, Seyer R, Bazin H, Ansanay H, Manning M, Mouillac B, Durroux T.
J Med Chem. 2007;50(20):4976-85. 
Fluorescent non-peptidic antagonists for V1a-R: Application to Tag-lite® technology and GPCR oligomerization assays using HTRF
Thiéric Rodriguez T, Pflimlin E, Ferrasse A, Hounsou C, Loison S, Wolf E, Lamarque L, Dupuis E, Hibert M, Durroux T, Bonnet D
5th HTRF Symposium, Avignon, France
Can HTRF® technology answer to different scientific questions? An academic with industrial standards point of view
Pascal Villa (PCBIS)
5th HTRF Symposium, Avignon, France-
Contribution of HTRF technology in the search for kinase inhibitors and GPCR ligands: the use of KinEASE and Tag-Lite assays
Villa P
MipTec 2012, Basel, Switzerland. Cisbio Workshop,
Scientific paper2
Title
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-
Time-resolved FRET between GPCR ligands reveals oligomers in native tissues.
Albizu L, Cottet M, Kralikova M, Stoev S, Seyer R, Brabet I, Roux T, Bazin H, Bourrier E, Lamarque L, Breton C, Rives ML, Newman A, Javitch J, Trinquet E, Manning M, Pin JP, Mouillac B, Durroux T.
Nat Chem Biol. 2010;6(8):587-94. -
Toward efficient drug screening by homogeneous assays based on the development of new fluorescent vasopressin and oxytocin receptor ligands.
Albizu L, Teppaz G, Seyer R, Bazin H, Ansanay H, Manning M, Mouillac B, Durroux T.
J Med Chem. 2007;50(20):4976-85.
Poster1
Title
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-
Fluorescent non-peptidic antagonists for V1a-R: Application to Tag-lite® technology and GPCR oligomerization assays using HTRF
Thiéric Rodriguez T, Pflimlin E, Ferrasse A, Hounsou C, Loison S, Wolf E, Lamarque L, Dupuis E, Hibert M, Durroux T, Bonnet D
5th HTRF Symposium, Avignon, France
Flyer1
Title
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Presentation2
Title
Related products
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-
Can HTRF® technology answer to different scientific questions? An academic with industrial standards point of view
Pascal Villa (PCBIS)
5th HTRF Symposium, Avignon, France -
Contribution of HTRF technology in the search for kinase inhibitors and GPCR ligands: the use of KinEASE and Tag-Lite assays
Villa P
MipTec 2012, Basel, Switzerland. Cisbio Workshop,