Ubiquitin toobox, a line of HTRF® reagents for monitoring protein ubiquitination.
Ubiquitination plays a key role in regulating protein degradation, and is carried out by the conjugation of ubiquitin to target proteins through the sequential activity of three enzymes: E1, E2 and E3. Poly-ubiquitinated proteins are then subject to degradation by proteasomes. The ubiquitin pathway is associated with many pathologies such as cancer, muscle atrophy, inflammation, viral infection, and metabolic and neurodegenerative disorders. Cisbio Bioassays offers a comprehensive line of HTRF® reagents for investigating ubiquitin pathway targets. The HTRF® Ubiquitin Toolbox assembles all the reagents necessary for flexible assay development and scale up to HTS, assessing each step in the ubiquitin pathway.