Tag-lite Toolbox is the result of the combination of HTRF with SNAP-tag®, CLIP-tag® and HaloTag® technologies. Tag-lite offers an unprecedented freedom and flexibility to built up your assays from target construction to assay development. Cisbio has optimized a selection of plasmids and fluorescent substrates that enable researchers to develop the assays they want in multiple assay configurations.
A plasmid construction is engineered with Tag-lite plasmids and the gene encoding your protein of interest. Once transfected into cells, this plasmid leads to the expression of a protein fused with the tag. The tagged protein covalently and specifically interacts with Tag-lite substrates.
Originally developed by the Ecole Polytechnique Federale de Lausanne (EPFL) and commercialized by New England Biolabs, Inc., SNAP-tag and CLIP-tag are small fusion tags that covalently interact with dyes conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. HaloTag, a Promega technology, is another orthogonal system for labeling proteins in living cells and in vitro.