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Human IgG Kappa & Lambda MAb assays

The use of human IgGs as therapeutic agents has increased recently, and Drug Discovery and Bioprocess now require their quantification.

Cisbio Bioassays offers assays to detect whole human antibodies selectively for Kappa and Lambda light chains. The Human IgG Kappa and Lambda MAb assays, based on HTRF® technology, are the right solutions for quantification, screening, and quality control.

Features

  • Simple and rapid assay protocol
  • Highly sensitive & accurate assay
  • Amenable to automation
  • Easily miniaturized
  • Compatible with culture media and additives

Applications

  • Selective screening of IgG κ and λ light chains
  • Specific to different types of IgG (1, 2, 3, and 4)
  • Screening of 100% human Ab
  • Bioprocess quality control

The HTRF Human IgG Kappa and Lambda MAb assays are sandwich immunoassays for the measurement of whole human IgGs selectively for Kappa and Lambda light chains. The assay uses a Eu3+-Cryptate conjugated antibody specific to human kappa or lambda light chains, and d2 coupled antibody specific to human Fc. This homogeneous assay enables precise assessment of all human IgG isotypes and requires only a single 2 hour incubation following the detection reagent addition.

Human IgG standard curve performed using protocol outlined in product insert.

  Kappa Lambda
Detection limit: 0.8 ng/mL 0.4 ng/mL
Dynamic range: 0.8 to 100 ng/mL 0.8 to 200 ng/mL
Specificity: subclasses IgG1, IgG2, IgG3 & IgG4

 

  Kappa Lambda
Cross reactivity 0% Human Lambda
0% Mouse Kappa
<1% Human IgM
0% Human Kappa
0% Mouse Lambda
0% Human IgM

Ordering Info

DescriptionCat. noProduct insertMSDS
Kappa MAb kit 1,000 tests64KAPPEB
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Kappa MAb kit 20,000 tests64KAPPEC
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Lambda MAb kit 1,000 tests64LAMPEB
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Lambda MAb kit 20,000 tests64LAMPEC
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Companion products

DescriptionCat. noProduct insertMSDS
Diluent 3 - 20 ml62DL3DDD
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Reconstitution buffer 3 - 13 ml62RB3RDD
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Reconstitution buffer 3 - 200 mL62RB3RDF
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