You are here : Home / Products / GPCRs / Cyclic-AMP & IP-One

Cyclic-AMP & IP-One

GPCR second messenger assays

Following activation, GPCRs transmit information within cells via the modulation of two second messengers, cyclic AMP for Gi/s coupled receptors and inositol-triphosphate (IP3) for Gq coupled receptors.

While cAMP accumulates in the presence of a phosphodiesterase inhibitor (e.g. IBMX), IP3 is unstable and rapidly enters the metabolic inositol phosphate cascade. However, in the presence of lithium chloride, IP1, a metabolic product of IP3, accumulates and offers a viable biomarker for the visualization of Gq-coupled receptor activity.

Features

  • Homogeneous, non-radioactive assays
  • Fast, robust and cost efficient alternative to ELISA kits

Applications

  • Gs, Gi or Gq coupled receptor activation
  • Assess agonists, antagonists, allosteric modulators and inverse agonists
  • Detection of slow acting agonist in functional assay
ask for a quote contact us

These competitive immunoassays are based on an IP1 or cAMP specific monoclonal antibody, labeled with a Terbium-cryptate. IP1 or cAMP produced by cells compete with d2-labeled IP1 or cAMP for binding to the antibody. Thus, the HTRF signal is inversely proportional to the level of cellular IP1 or cAMP present.

IP1 standard curve performed using protocol outlined in IP-One Tb assay product insert.

 

  S/B standard curve IC50 Specificity
cAMP dynamic 2 kit ≥15 3 to 5.6 nM No cross-reactivity with 500 nM ATP, ADP, cGMP
IP-One Tb kit ≥18 ≤140 nM No cross-reactivity with 50 µM Myo-inositol, PIP2, IP2, IP3, IP4 or PIP3




© 2013 Cisbio.com - All Rights Reserved