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Cyclic AMP cell-based assay kit

HTRF GPCR cAMP cell-based assay kit

Cyclic AMP:  the unique GPCR Gi/s second messenger accumulation assay

A line of three ready-to-use assays for measuring cyclic AMP using HTRF® technology. Each kit is optimized for the detection of a specific range of cAMP concentrations. These kits give high quality, cell-based assay screening in 96-, 384- or 1536-well formats.

Features

  • Cell-based functional assay
  • Second messenger accumulation assay
  • Assay formats 100 µL and 20 µL
  • Miniaturization down to < 10µl
  • Compatible with fresh or frozen cells

Applications

  • Gs or Gi coupled receptor activation
  • Assess agonists, antagonists, inverse agonists and allosteric modulators
  • Phosphodiesterase (PDE) monitoring
  • Adenylate cyclase activity assessment
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cAMP kits are based on a competitive immunoassay using cryptate-labeled anti-cAMP antibody and d2-labeled cAMP.

cAMP assay kit two step protocol, after cell stimulation

cAMP assay kit two step protocol after cell stimulation

 

Three kit groups to cover a broad range of cell-based assays.

cAMP femto kits

cAMP femto kits are the most sensitive cAMP cell-based assays. With the introduction of Lumi4®-Tb cryptate, cAMP femto is now available with two cryptates (europium or terbium). Both kits enable the detection of as little as 28 pM cAMP (0.6 fmoles/well in 20µl volume).


cAMP dynamic 2

cAMP dynamic 2 covers a wide range of cAMP concentrations. This kit is ideal for a broad range of Gi/s assays.


cAMP HiRange

With an extended signal-to-background ratio and Z' > 0.9, cAMP HiRange is streamlined for HTS. The antibody does not cross-react with ATP.

  Cryptate S/B IC20 nM* IC50 nM* IC80 nM*
cAMP femto Kit Eu3+ >8 0.33 1.76 9.4
cAMP femto Tb Kit Tb2+ >8 0.33 1.76 9.4
cAMP dynamic 2 Kit Eu3+ >14 0.91 4.07 18.1
cAMP HiRange Kit Eu3+ >28 4.27 22.8 121

* final cAMP concentration

cAMP assay kit  S/B vs concentration

 

Ordering Info

DescriptionCat. noProduct insertMSDS
cAMP dynamic 2 kit - 1,000 tests62AM4PEB
cAMP dynamic 2 kit - 20,000 tests62AM4PEC
cAMP dynamic 2 kit -2 X 96 tests62AM4PEF
cAMP dynamic 2 kit - 100,000 tests62AM4PEJ
cAMP dynamic 2 kit -1 M tests (10 packs 62AM4PEJ)62AM4PEM
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cAMP femto 2 kit - 1,000 tests62AM5PEB
cAMP femto 2 kit - 20,000 tests62AM5PEC
cAMP femto 2 kit - 100,000 tests62AM5PEJ
cAMP HiRange kit - 1,000 tests62AM6PEB
cAMP HiRange kit - 20,000 tests62AM6PEC
cAMP HiRange kit - 100,000 tests62AM6PEJ
cAMP HiRange kit - 1 M tests (10 packs 62AM6PEJ)62AM6PEM
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cAMP femto Tb kit - 1,000 tests62AM7PEB
cAMP femto Tb kit - 20,000 tests62AM7PEC
cAMP femto Tb kit - 100,000 tests62AM7PEJ

Companion products

DescriptionCat. noProduct insertMSDS
cAMP dynamic 2 calibrator 62AM4CDA
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cAMP femto 2 calibrator 62AM5CDA
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cAMP HiRange calibrator 62AM6CDA
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Lysis buffer 1 cAMP/cGMP kits 1,000 tests - 13 ml62CL1FDD
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Lysis buffer 2 cAMP/cGMP kits 20, & 100,000 tests62CL2FDF
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96 half-well white plate cell culture - 8 plates66PL8ZZZ
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