Custom assay development and HTS projects often require the labeling of specific assay components. Cisbio Bioassays offers services for the labeling of proteins, peptides, oligonucleotides and other biomolecules with HTRF fluorophores such as cryptates (Europium and Terbium), XL665 or d2. We also provide labeling services for biotin, DNP conjugation or other fluorescent dyes.
For all labeling types, test and batch size options are available, offering the scientist flexibility and maximum productivity. Our team of experienced scientists provides high quality labeling with personalized customer support.
Material to be labeled can be provided by the customer or sourced from an external vendor by Cisbio Bioassays. Consultation with our staff is provided to develop a labeling approach that addresses the specific requirements of the assay. During the labeling process, our scientists continuously monitor sample integrity to ensure quality. Following labeling, the sample is fully characterized to provide our customers with the appropriate working and quality control information.
This option is ideal for researchers who have a valuable sample, want to assess labeling feasibility and efficiency, or are looking to label a small quantity of sample for assay development. Our scientists carry out a small size labeling that generally produces enough material for customers to perform at least 1,000 assay points. This service enables researchers to test the functional performance of a labeled biomolecule in their assay at a lower cost and prior to scaling up to batch size labeling. The chemistry and processes optimized during test labeling can be applied to batch labeling protocols during scale-up, meaning a streamlined migration from small-scale to large-scale labeling.
This service provides custom labeling of large milligram quantities of biomolecule.
Note: Cryptate custom-labeled products are calibrated using a PHERAstar plate reader (BMG-Labtech). Delivered quantity of the cryptate conjugate is designated as the number of 20µl (final volume) assay points that can be run in a 384-well low volume plate. For XL665, d2, biotin and DNP labelings, delivered quantities of proteins are designated in milligram units, and oligonucleotides are supplied based on OD units.
For the pricing and turn around time estimates for your specific project, please contact your local sales representatives.
|XL665||d2||Biotin||DNP||Other fluo dye|
|Test labeling package||64CUSKAY||64CUSTAY||64CUSXAY||64CUSDAY||64CUSBAY||64CUSNAY||64CUSFAY|
|Batch labeling package||64CUSKAZ||64CUSTAZ||64CUSXAZ||64CUSDAZ||64CUSBAZ||64CUSNAZ||64CUSFAZ|
|Tag-lite® custom-labeled cells||CELLCUST|
|Ligand green custom labeling||LCUSTGRE|
|Ligand red custom labeling||LCUSTRED|
|Ligand terbium custom labeling||LCUSTTER|