HTRF® Compatible Readers

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HTRF® technology requires simple but specific detection conditions. The HTRF® signal is detected at two different wavelengths (typically 620 nm and 665 nm) which are used to calculate the fluorescence ratio and compensate for compound interference and sample quenching. Emissions are also detected using a time-resolved module which enables the introduction of a delay between the excitation pulse and the time-gated measurement window that greatly diminishes background fluorescence detection.

Optical devices such as excitation and emission filters, monochromators and dichroic mirrors, as well as energy source and photomultiplier tubes, constitute critical equipment for measuring HTRF® output. Instrument set-up has a significant impact on detection efficiency, in particular the measurement window and time delay, excitation parameters (e.g. number of flashes delivered), gain adjustment, and reader head positioning with respect to the well. The reader control kit can be used to assess the performance of compatible readers to read HTRF® technology using either Europium or Terbium cryptate chemistry.

In order to broaden HTRF® readout capabilities for use in the field of life sciences, Cisbio Bioassays has initiated partnerships with several detection system manufacturers. This collaboration has lead to reader certification, signified by an HTRF® sticker. This label guarantees that the reader meets the physical specifications for optimal HTRF® readout.


The following instruments have already been certified for HTRF® readout:

RUBYstar
PHERAstar
Safire2™
Infinite™ F500
FlexStation® 3
Spectramax M5e
Analyst GT
Analyst AD & HT
ScreenStation HT
Artemis 101
Synergy™ 4
Synergy™ 2
PARADIGM™ Detection Platform
Mithras LB 940

You may also check the compatibility of your equipment by contacting your local Cisbio Bioassays sales and application support team.


HTRF® reader control kit: a straightforward procedure for reader check-up

The HTRF® reader control kit is designed for calibrating all HTRF® compatible readers using either Europium or Terbium cryptate chemistry. This immunoassay-based kit includes all the necessary reagents (conjugates and standards) as well as buffers and one 96 half-well microplate. The plate map includes 2 assays (Eu3+ and Tb2+ assays) using a low and a high level standard, conjugate and buffer blanks. After 1 hour incubation at room temperature (18-25°C) the plate can be read by using the appropriate wavelenght for Europium and Terbium (when available).

Data reduction can be processed by using the Microsoft EXCEL file 62RCKPEA_appendix.xls which will deliver the reader QC report. The same plate (i.e. experiment) may be used to check several instruments.

This kit may also be used to validate readers following their upgrade to HTRF® compatibility. It provides an easy and straightforward way to check the overall performance of a reader and may facilitate the identification of potential failures.

Description Quantity Cat no. Product Insert
HTRF® reader control kit 1 kit 62RCKPEA pdf
Reader control kit QC report 1 kit 62RCKPEA_appendix xls