HTRF Products

• GPCR Solutions
• Kinase & Oncology
• Inflamation Metabolic Diseases, CNS
• Bioprocess
• Labeling
• Anti-tag
• Streptavidins
• Toolbox Reagents

Services

• Custom Labeling
• Assay Development
• Training

Sandwich assays:

• Sandwich assays can only be used when the molecule of interest has two distinct binding sites for antibodies.
• HTRF® sandwich assays use two antibodies coupled to Eu3+ or Tb2+ Cryptate and to an acceptor (i.e. XL665 or d2).
• The two conjugates bind to the antigen when present in the sample, thereby generating FRET. Signal intensity is proportional to the number of Ab-Ag complexes formed, and therefore to Ag concentration.
• The specific signal modulates positively in proportion to Ag concentration.

Immunocompetitive assays:

• These assays are designed to assess molecules that are too small to allow the binding of two antibodies.
• The assay components consist of an acceptor - conjugate generally made of purified antigen, and its respective Ab coupled to Eu3+ or Tb2+ Cryptate.
• The Ag present in the sample competes for binding with the labeled antibody and thereby prevents FRET from occurring.
• FRET intensity decreases with the amount of dissociated Ab-Ag complexes (i.e. inversely proportional to Ag concentration)
• The specific signal modulates negatively, inversely proportional to Ag concentration

HTRF® Immunoassays