HTRF® Microplate Recommendations

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Photo courtesy of Greiner

Most available microplate brands are compatible with HTRF® technology and products. Nevertheless, background levels may differ substantially among various plate types. FIA black plates from many suppliers remain the most appropriate standard. Tissue-culture treated, clear-bottom, or surface-treated plates may also be used. Glass bottom plates produce suboptimal results and should not be used. The following plates produced excellent results during our validation process for a broad range of HTRF® assays:




Provider Format Recommended volume Color Cat no.
Greiner 384-well 80-100 µL Black 781076
Greiner 384-well 80-100 µL White 781075
Greiner 384 low-volume 8-20 µL Black 784076
Greiner 384 low-volume 8-20 µL White 784075
Greiner 96-well 160-200 µL White - Tissue-culture treated sterile 655083
Greiner 384-well 80-100 µL White - Tissue-culture treated sterile 781080
Greiner 384 low volume 8-20 µL White - Tissue-culture treated sterile 784080
Greiner 384 low-volume 8-20 µL White 784075
Corning 96-well 160-200 µL Black 3915
Corning 96-well 160-200 µL White 3912
Corning 96 half-area 80-100 µL Black 3694
Corning 96 half-area 80-100 µL White 3693

Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam allows maximum energy delivery. For instance, when assay volumes are equal to, or lower than, 20 µl, it is better to use 384 low-volume or 1536-well plates rather than regular 384-well plates.

The signal to noise ratios obtained using white microplates do not differ greatly from those of black plates. Absolute counts, however, are significantly higher in white plates, thereby improving count statistics and subsequent assay reproducibility and sensitivity. NOTE: The use of white plate is mandatory for some assays such as IP-One, and highly recommended when detection is performed using certain instruments, in particular on monochromator-based plate readers