HTRF^® Technology

The Genuine TR-FRET Chemistry
HTRF^® (homogeneous time resolved fluorescence) is a technology based on TR-FRET, a combination of FRET chemistry and the use of fluorophores with long emission half-lives. While HTRF is based on TR-FRET chemistry it has many properties that separate it from other TR-FRET products. These include the use of Europium and Terbium cryptate chemistries. The lanthanides have a long life and their complexing to macrocycles to form cryptates allows increased stability. Finally, the use of a patented radiometric measurement enables correction for quenching and sample interferences.

	TR-FRET Basics			Cell-based Assays
	HTRF Chemistry			Immunoassays

	Signal Stability			Assay Miniaturization
	Compatible Readers			Toolbox Reagent Usage
	HTRF Ratio and Data Reduction			Microplate Recommendations

Description and Applications