The other flexible HTRF® assay development platform
Tag-lite Toolbox is the result of the combination of HTRF with SNAP-tag®, CLIP-tag® and HaloTag® technologies. Tag-lite offers an unprecedented freedom and flexibility to built up your assays from target construction to assay development. Cisbio has optimized a selection of plasmids and fluorescent substrates that enable researchers to develop the assays they want in multiple assay configurations.
A plasmid construction is engineered with Tag-lite plasmids and the gene encoding your protein of interest. Once transfected into cells, this plasmid leads to the expression of a protein fused with the tag. The tagged protein covalently and specifically interacts with Tag-lite substrates.
Originally developed by the Ecole Polytechnique Federale de Lausanne (EPFL) and commercialized by New England Biolabs, Inc., SNAP-tag and CLIP-tag are small fusion tags that covalently interact with dyes conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. HaloTag, a Promega technology, is another orthogonal system for labeling proteins in living cells and in vitro.
- Cisbio Bioassays proposes plasmids encoding SNAP-tag, CLIP-tag, HaloTag and bordered by restriction sites for cloning a gene of interest.
- By using these plasmids, a construction can be engineered.
- This construction encodes for the protein of interest and SNAP-tag, CLIP-tag or HaloTag in the N or C terminal position.
- A selection of three substrates is proposed for each SNAP-tag, CLIP-tag and HaloTag.
- These substrates are labeled with HTRF fluorophores, terbium cryptate donor (Lumi4-Tb), and green or red HTRF acceptors.