- Did you know Cisbio Bioassays offers custom labeling services?
"I have chosen to outsource my assay development because HTRF is a very common technology across the drug discovery world. Cisbio was able to deliver exactly what I needed on time and with high quality. For these reasons I have chosen to work with Cisbio on seven projects over the past two years."
Jun Xian, Ph.D.
Associate Director, Partners Center for Drug Discovery
Brigham & Women's Hospital
Harvard Medical School
Assay development
Dedicated to your results
Cisbio Bioassays offers assay development services for the creation and/or optimization of HTRF® assays. Our dedicated scientists are experts in TR-FRET technology and in assay design for a wide-range of assay types.
Assay development is customized to meet the individual needs of each individual and is performed under strict confidentiality guidelines. You can choose our two or four step process to maximize efficiency. Either option results in the same high-quality work.
Advantages of choosing Cisbio Bioassays assay development services
- Full confidentiality maintained at all times
- Direct point-of-contact with a dedicated scientific consultant
- Tailored assay development approach
- Personnel highly trained in protein chemistry and fluorescence methodologies
- State-of-art facilities in US, Europe and Japan with cell culture capabilities
- Experience in a wide range of applications
- On-site assay knowledge transfer available
Case study
A repeat customer made a request for us to fully develop and validate an assay to measure MAP4K5 (KHS1) activity. In addition to the assay development, we cryptate labeled the anti-phospho antibody and procured a biotinylated substrate to allow for the use of Streptavidin-XL665 for detection.
The assay was to be utilized in high-throughput screening to identify modulators of the enzyme's activity. With this in mind, a plan for assay development was put in place and communicated to the customer. This included the kinetic characterization of the enzyme with respect to rate, Km of ATP and substrate as well as inhibitor profiling. Further, the stability of the reagents diluted over time and the statistical robustness of the data was assessed to ensure that the assay would be suitable for high-throughput screening. Following the complete validation, a report was generated and the assay was transferred to the customer.
Independent IC50 determinations for staurosporine demonstrated that the assay was able to give reproducible results, falling in the literature reported range of activity for the inhibitor. Further, the IC50 value generated with enzyme diluted over three hours was in line with those values generated using freshly prepared material. This is indicative of the stability and robustness of the assay.
Statistical data for various experiments indicate that the assay is reproducible as well as statistically robust. The S/B and z' ratios indicate that the screen should perform well in an automation setting.
Our unique and flexible solution:
Under strict confidentiality and constant communication with a dedicated Project Manager, assay development projects are generally completed within 4 to 8 weeks.
Best pair package
Start your assay development with the optimal reagents
Includes at a minimum:
- Reagent labeling
- Best pair analysis
- 5,000 tests of best pair
Full assay development package
Full customized solution developed in collaboration with scientist
Includes at a minimum:
- Literature-based assay feasibility study
- Reagent labeling
- Best pair analysis
- Optimization of assay parameters (i.e.: time course, Km, sensitivity, z')
- Technical report
- 5,000 tests delivered
Ordering Info:
For pricing and turn around time estimates for your specific project please contact your local sales representatives.
| Designation | Part number |
| Best pair package | 63BPP000 |
| Paper study only | 63PAP000 |
| Full Assay development package | 63ADR000 |
For more information or To Place an Order:
U.S. and Canada
1-888-963-4567
Europe and other countries
+33(0)466 796 705
Japan
+81-3-5510-2932
