HTRF Resource Library

Ultra high throughput GPCR screening utilizing CCD imaging systems with HTRF®

By:  S. Feighner

Merck

Abstract:

The Merck Rahway Facility for Automation and Screening Technologies (RY-FAST) is a group with three main functions: a.) compound management for lead optimization programs,  b.) provide assay support for medicinal chemistry programs and c.) function as a liason between the uHTS group and therapeutic groups by miniaturizing assays to 1536 and 3456 well formats.  This presentation will outline the steps to define a robust protocol for a Gs-coupled GPCR from 384 well format through 1536 well and up to 3456 well microtiter plates using a Viewlux multimode CCD-based plate reader.