HTRF Resource Library

Development of an IL5 HTRF® assay for high throughput screening of primary human T cells

By: Jennifer Brogdon

Novartis

Abstract:
The abberant production of T cell cytokines can lead to autoimmunity or asthma. Identification of small molecule inhibitors for cytokine production can be challenging due to the lack of appropriate cell lines which mimic T cell cytokine regulation. We developed an HTRF-based method to measure cytokine production from primary human T cells and adapted the assay to automation for ultra high throughput screening in 1536 well format. Assay development, optimization and screening of primary cells will be discussed.