HTRF Resource Library

The use of HTRF® in an automated workflow for Human MAb screening at Medarex

By: Denise Williams

Medarex

Abstract

Screening for monoclonal antibodies at Medarex traditionally involved the use of ELISA which included several time consuming steps not ideal for automation.  Due to our throughput requirements and the need to standardize for increased efficiency, we have developed an automated high throughput screening system at Medarex which utilizes an HTRF® assay that works flawlessly integrated with our custom robotics and data management system.  Our simple and robust HTRF® assay uses commercially available antibodies which are custom labeled for us by Cisbio.  The decision to include the HTRF® in our automated process has enabled us to screen a much higher number of plates and has shown a steady increase in our antibody output over the last several years.  

Medarex is using the HTRF assay in other areas of research and development as well. As a service to our research department, we use the HTRF assay to quantitate the antibody concentration in our Hybridoma supernatants once hits are identified and have also transferred our knowledge to our cell line development group where our lead candidates are transfected into CHO cell lines.