HTRF Resource Library

Application of TR-FRET Systems in Kinome Profiling, Mechanistic Studies, and as an Alternative to Conventional Fluorescence Polarization

By: Eric Johnson

Abbott

Abstract:

Rationale and strategy for kinome profiling including inhibitor and kinase selection, HTRF vs 33P-based platform comparison, and assay development will be discussed.  Examples of mechanistic studies on kinase inhibitors utilizing the HTRF platform will be given.  The development of TR-FRET binding assays to replace/compliment conventional fluorescence polarization methods and the increased utility of the TR-FRET method will be described.