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Scientific paper169

• High throughput screening of beta-amyloid secretion inhibitors using homogenous time-resolved fluorescence
  Albrecht H, Zbinden P, Rizzi A, Villetti G, Riccardi B, Puccini P, Catinella S, Imbimbo BP
  Comb Chem High Throughput Screen. 2004 Dec;7(8):745-56.
  Publication year: 2013

  HTRF® Anti-species reagents toolbox

  Streptavidin & affinity reagents toolbox
• Rapid sensing of circulating ghrelin by hypothalamic appetite-modifying neurons
  Schaeffer M, Langlet F, Lafont C, Molino F, Hodson DJ, Roux T, Lamarque L, Verdié P, Bourrier E, Dehouck B, Banères JL, Martinez J, Méry PF, Marie J, Trinquet E, Fehrentz JA, Prévot V, Mollard P.
  Proc Natl Acad Sci U S A. 2013;110(4):1512-7.
  Publication year: 2013

  Ghrelin GHSR1A receptor ligand binding assay
• Illuminating the activation mechanisms and allosteric properties of metabotropic glutamate receptors
  Doumazane E, Scholler P, Fabre L, Zwier JM, Trinquet E, Pin JP, Rondard P.
  Proc Natl Acad Sci U S A. 2013.
  Publication year: 2013

  IP-One HTRF® assay

  Tag-lite® substrates

  
• High-precision, room temperature screening assay for inhibitors of microsomal prostaglandin E synthase-1
  Andersson S, Norman M, Olsson R, Smith R, Liu G, Nord J.
  J Biomol Screen. 2012;17(10):1372-8.
  Publication year: 2012

  Human & Mouse TNFα assays
• Characterization of a Ca2+/calmodulin-dependent AC1 adenylyl cyclase in a non-neuronal tissue, the blowfly salivary gland
  
  Cell Calcium. 2012;52(2):103-12.
  Publication year: 2012

  cAMP cell-based assay
• Development and validation of a generic fluorescent methyltransferase activity assay based on the transcreener AMP/GMP assay
  Klink TA, Staeben M, Twesten K, Kopp AL, Kumar M, Dunn RS, Pinchard CA, Kleman-Leyer KM, Klumpp M, Lowery RG
  J Biomol Screen. 2012 ;17(1):59-70.
  Publication year: 2012

  Transcreener® EPIGEN Methyltransferase Assay
• Analysis of ubiquitin E3 ligase activity using selective polyubiquitin binding proteins.
  Marblestone JG, Larocque JP, Mattern MR, Leach CA.
  Biochim Biophys Acta. 2012;1823(11):2094-7.
  Publication year: 2012

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox
• Functional analysis of TCF4 missense mutations that cause Pitt-Hopkins syndrome
  Forrest M, Chapman RM, Doyle AM, Tinsley CL, Waite A, Blake DJ.
  Hum Mutat. 2012 Jul 6. doi: 10.1002/humu.22160
  Publication year: 2012

  HTRF® Anti-tag reagents toolbox
• CASR gene activating mutations in two families with autosomal dominant hypocalcemia
  Guarnieri V, Valentina D'Elia A, Baorda F, Pazienza V, Benegiamo G, Stanziale P, Copetti M, Battista C, Grimaldi F, Damante G, Pellegrini F, D'Agruma L, Zelante L, Carella M, Scillitani A.
  Mol Genet Metab. 2012 Jun 26.
  Publication year: 2012

  IP-One ELISA assay
• Allosteric Modulation of Endogenous Metabolites as an Avenue for Drug Discovery
  Wootten D, Savage EE, Valant C, May LT, Sloop KW, Ficorilli J, Showalter AD, Willard FS, Christopoulos A, Sexton PM.
  Mol Pharmacol. 2012;82(2):281-90.
  Publication year: 2012

  Insulin assay
• Synthesis and evaluation of debromohymenialdisine-derived Chk2 inhibitors
  Saleem RS, Lansdell TA, Tepe JJ.
  Bioorg Med Chem. 2012;20(4):1475-81.
  Publication year: 2012

  HTRF® KinEASE™
• The mechanism of action of doxofylline is unrelated to HDAC inhibition, PDE inhibition or adenosine receptor antagonism
  van Mastbergen J, Jolas T, Allegra L, Page CP
  Pulm Pharmacol Ther. 2012 Feb;25(1):55-61
  Publication year: 2012

  cGMP assay

  cAMP cell-based assay
• Detecting S-adenosyl-l-methionine-induced conformational change of a histone methyltransferase using a homogeneous time-resolved fluorescence-based binding assay Detecting S-adenosyl-l-methionine-induced conformational change of a histone methyltransfera
  Lin Y, Fan H, Frederiksen M, Zhao K, Jiang L, Wang Z, Zhou S, Guo W, Gao J, Li S, Harrington E, Meier P, Scheufler C, Xu YC, Atadja P, Lu C, Li E, Gu XJ.
  Anal Biochem. 2012;423(1):171-7.
  Publication year: 2012

  HTRF® Anti-tag reagents toolbox
• Synthesis and biological evaluation of quinazoline and quinoline bearing 2,2,6,6-tetramethylpiperidine-N-oxyl as potential epidermal growth factor receptor(EGFR) tyrosine kinase inhibitors and EPR bio-probe agents
  Li S, Guo C, Sun X, Li Y, Zhao H, Zhan D, Lan M, Tang Y
  Eur J Med Chem. 2012;49C:271-8.
  Publication year: 2012

  HTRF® KinEASE™
• Rare MTNR1B variants impairing melatonin receptor 1B function contribute to type 2 diabetes
  Bonnefond A, Clément N, Fawcett K, Yengo L, Vaillant E, Guillaume JL, Dechaume A, Payne F, Roussel R, Czernichow S, Hercberg S, Hadjadj S, Balkau B, Marre M, Lantieri O, Langenberg C, Bouatia-Naji N; The Meta-Analysis of Glucose and Insulin-Related Traits Consortium (MAGIC), Charpentier G, Vaxillaire M, Rocheleau G, Wareham NJ, Sladek R, McCarthy MI, Dina C, Barroso I, Jockers R, Froguel P.
  Nat Genet. 2012 Jan 29. doi: 10.1038/ng.1053.
  Publication year: 2012

  IP-One HTRF® assay
• Apo-Ghrelin Receptor Forms Heteromers with DRD2 in Hypothalamic Neurons and Is Essential for Anorexigenic Effects of DRD2 Agonism
  Kern A, Albarran-Zeckler R, Walsh HE, Smith RG.
  Neuron. 2012 Jan 26;73(2):317-32.
  Publication year: 2012

  Tag-lite® toolbox
• Biased agonism of the calcium-sensing receptor
  Thomsen AR, Hvidtfeldt M, Bräuner-Osborne H.
  Cell Calcium. 2012;51(2):107-16.
  Publication year: 2012

  cAMP cell-based assay

  IP-One HTRF® assay

  Phospho-ERK (Cellul'erk)

  
• Synthesis and biological evaluation of 4-[3-chloro-4-(3-fluorobenzyloxy)anilino]-6-(3-substituted-phenoxy)pyrimidines as dual EGFR/ErbB-2 kinase inhibitors
  Li S, Guo C, Zhao H, Tang Y, Lan M.
  Bioorg Med Chem. 2012;20(2):877-85.
  Publication year: 2012

  HTRF® KinEASE™
• Excessive Food Intake, Obesity and Inflammation Process in Zucker fa/fa Rat Pancreatic Islets
  Chentouf M, Dubois G, Jahannaut C, Castex F, Lajoix AD, Gross R, Peraldi-Roux S
  PLoS One. 2011;6(8):e22954
  Publication year: 2011

  Insulin assay
• Upregulation of β-cell genes and improved function in rodent islets following chronic glucokinase activation
  Gill D, Brocklehurst KJ, Brown HW, Smith DM
  J Mol Endocrinol. 2011 Jul 18;47(1):59-67
  Publication year: 2011

  Insulin assay
• Structure of the Ire1 autophosphorylation complex and implications for the unfolded protein response
  Ali MM, Bagratuni T, Davenport EL, Nowak PR, Silva-Santisteban MC, Hardcastle A, McAndrews C, Rowlands MG, Morgan GJ, Aherne W, Collins I, Davies FE, Pearl LH.
  
  Publication year: 2011

  HTRF® KinEASE™
• TBK1 directly engages Akt/PKB survival signaling to support oncogenic transformation
  Ou YH, Torres M, Ram R, Formstecher E, Roland C, Cheng T, Brekken R, Wurz R, Tasker A, Polverino T, Tan SL, White MA.
  Mol Cell. 2011;41(4):458-70.
  Publication year: 2011

  HTRF® KinEASE™
• Synthesis and biological evaluation of 7-substituted-1-(3-bromophenylamino)isoquinoline-4-carbonitriles as inhibitors of myosin light chain kinase and epidermal growth factor receptor
  Rode HB, Sos ML, Grütter C, Heynck S, Simard JR, Rauh D.
  Bioorg Med Chem. 2011;19(1):429-39.
  Publication year: 2011

  HTRF® KinEASE™
• Drug to genome to drug: discovery of new antiplasmodial compounds
  Beghyn TB, Charton J, Leroux F, Laconde G, Bourin A, Cos P, Maes L, Deprez B.
  J Med Chem. 2011;54(9):3222-40
  Publication year: 2011

  cAMP cell-based assay

  cGMP assay
• Two High Throughput Screen Assays for Measurement of TNF-α in THP-1 Cells
  Leister KP, Huang R, Goodwin BL, Chen A, Austin CP, Xia M.
  Curr Chem Genomics. 2011;5:21-9.
  Publication year: 2011

  Human & Mouse TNFα assays
• Mouse monoclonal antibodies to anthrax edema factor protect against infection
  Leysath CE, Chen KH, Moayeri M, Crown D, Fattah R, Chen Z, Das SR, Purcell RH, Leppla SH.
  Infect Immun. 2011 Nov;79(11):4609-16.
  Publication year: 2011

  cAMP cell-based assay

  Antibody mechanism of action study
• Whole-Molecule Antibody Engineering: Generation of a High-Affinity Anti-IL-6 Antibody with Extended Pharmacokinetics
  Finch DK, Sleeman MA, Moisan J, Ferraro F, Botterell S, Campbell J, Cochrane D, Cruwys S, England E, Lane S, Rendall E, Sinha M, Walker C, Rees G, Bowen MA, Schneider A, Liang M, Faggioni R, Fung M, Mallinder PR, Wilkinson T, Kolbeck R, Vaughan T, Lowe DC.
  J Mol Biol. 2011;411(4):791-807
  Publication year: 2011

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox

  Biochemical targets

  
• The orexin OX1 receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands.
  Xu TR, Ward RJ, Pediani JD, Milligan G
  Biochem J. 2011;439(1):171-83.
  Publication year: 2011

  Tag-lite® toolbox

  Tag-lite® plasmids
• Investigation of prolactin receptor activation and blockade using time-resolved fluorescence resonance energy transfer.
  Tallet E et all
  Frontiers in endocrinology. 2011,2(29): 1-9
  Publication year: 2011

  Tag-lite® toolbox
• Monitoring Gq-coupled receptor response through inositol phosphate quantification with the IP-One assay.
  Trinquet E, Bouhelal R, Dietz M
  Expert Opin. Drug Discov. (2011);6(10):981-994.
  Publication year: 2011

  IP-One HTRF® assay
• Dual inhibitory effect of Glycyrrhiza glabra (GutGard™) on COX and LOX products.
  Chandrasekaran CV, Deepak HB, Thiyagarajan P, Kathiresan S, Sangli GK, Deepak M, Agarwal A.
  Phytomedicine. 2011;18(4):278-84.
  Publication year: 2011

  Prostaglandin E2 assay

  Leukotriene B4 & Leukotriene C4 assays
• Ligand-induced internalization of the orexin OX(1) and cannabinoid CB(1) receptors assessed via N-terminal SNAP and CLIP-tagging.
  Ward RJ, Pediani JD, Milligan G.
  Br J Pharmacol. 2011;162(6):1439-52.
  Publication year: 2011

  Tag-lite® toolbox
• Momordica charantia extract, a herbal remedy for type 2 diabetes, contains a specific 11β-hydroxysteroid dehydrogenase type 1 inhibitor.
  Blum A, Loerz C, Martin HJ, Staab-Weijnitz CA, Maser E
  J Steroid Biochem Mol Biol. 2012;128(1-2):51-5.
  Publication year: 2011

  Cortisol assay

  
• Identification of cyclicsulfonamide derivatives with an acetamide group as 11β-hydroxysteroid dehydrogenase 1 inhibitors
  Kim SH, Kwon SW, Chu SY, Lee JH, Narsaiah B, Kim CH, Kang SK, Kang NS, Rhee SD, Bae MA, Ahn SH, Ha DC, Kim KY, Ahn JH
  Chem Pharm Bull (Tokyo). 2011;59(1):46-52
  Publication year: 2011

  Cortisol assay
• Discovery of the first non-ATP competitive IGF-1R kinase inhibitors: advantages in comparison with competitive inhibitors
  Lesuisse D, Mauger J, Nemecek C, Maignan S, Boiziau J, Harlow G, Hittinger A, Ruf S, Strobel H, Nair A, Ritter K, Malleron JL, Dagallier A, El-Ahmad Y, Guilloteau JP, Guizani H, Bouchard H, Venot C.
  Bioorg Med Chem Lett. 2011 Apr 15;21(8):2224-8.
  Publication year: 2011

  Kinase toolbox reagents
• Biomimetic screening of class-B G protein-coupled receptors
  Devigny C, Perez-Balderas F, Hoogeland B, Cuboni S, Wachtel R, Mauch CP, Webb KJ, Deussing JM, Hausch F
  J Am Chem Soc. 2011 Jun 15;133(23):8927-33
  Publication year: 2011

  cAMP cell-based assay
• Developing Chemical Genetic Approaches to Explore G Protein-Coupled Receptor Function: Validation of the Use of a Receptor Activated Solely by Synthetic Ligand (RASSL)
  Alvarez-Curto E, Prihandoko R, Tautermann CS, Zwier JM, Pediani JD, Lohse MJ, Hoffmann C, Tobin AB, Milligan G
  Mol Pharmacol. 2011 Dec;80(6):1033-46
  Publication year: 2011

  Tag-lite® toolbox
• Inhibition of morphine-induced cAMP overshoot: a cell-based assay model in a high-throughput format
  Xia M, Guo V, Huang R, Shahane SA, Austin CP, Nirenberg M, Sharma SK.
  Cell Mol Neurobiol. 2011 Aug;31(6):901-7.
  Publication year: 2011

  cAMP cell-based assay
• G protein activation by serotonin type 4 receptor dimers: evidence that turning on two protomers is more efficient
  Pellissier LP, Barthet G, Gaven F, Cassier E, Trinquet E, Pin JP, Marin P, Dumuis A, Bockaert J, Banères JL, Claeysen S.
  J Biol Chem. 2011 Mar 25;286(12):9985-97.
  Publication year: 2011

  cAMP cell-based assay

  IP-One HTRF® assay
• Selective orthosteric free fatty acid receptor 2 (FFA2) agonists: identification of the structural and chemical requirements for selective activation of FFA2 versus FFA3
  Schmidt J, Smith NJ, Christiansen E, Tikhonova IG, Grundmann M, Hudson BD, Ward RJ, Drewke C, Milligan G, Kostenis E, Ulven T
  J Biol Chem. 2011;286(12):10628-40.
  Publication year: 2011

  cAMP cell-based assay

  IP-One HTRF® assay
• THRX-198321 is a bifunctional muscarinic receptor antagonist and beta2-adrenoceptor agonist (MABA) that binds in a bimodal and multivalent manner
  Steinfeld T, Hughes AD, Klein U, Smith JA, Mammen M.
  Mol Pharmacol. 2011;79(3):389-99.
  Publication year: 2011

  cAMP cell-based assay

  IP-One HTRF® assay
• Discovery of a second generation agonist of the orphan G-protein coupled receptor GPR119 with an improved profile
  Semple G, Lehmann J, Wong A, Ren A, Bruce M, Shin YJ, Sage CR, Morgan M, Chen WC, Sebring K, Chu ZL, Leonard JN, Al-Shamma H, Grottick AJ, Du F, Liang Y, Demarest K, Jones RM.
  Bioorg Med Chem Lett. 2011 Dec 30.
  Publication year: 2011

  cAMP cell-based assay
• Drug discovery using chemical systems biology: weak inhibition of multiple kinases may contribute to the anti-cancer effect of nelfinavir
  Xie L, Evangelidis T, Xie L, Bourne PE.
  PLoS Comput Biol. 2011;7(4):e1002037.
  Publication year: 2011

  HTRF® Transcreener® ADP
• Benzimidazolones: a new class of selective peroxisome proliferator-activated receptor γ (PPARγ) modulators
  Liu W, Lau F, Liu K, Wood HB, Zhou G, Chen Y, Li Y, Akiyama TE, Castriota G, Einstein M, Wang C, McCann ME, Doebber TW, Wu M, Chang CH, McNamara L, McKeever B, Mosley RT, Berger JP, Meinke PT.
  J Med Chem. 2011;54(24):8541-54.
  Publication year: 2011

  HTRF® Anti-tag reagents toolbox
• A therapeutic antibody targeting BACE1 inhibits amyloid-β production in vivo.
  Atwal JK, Chen Y, Chiu C, Mortensen DL, Meilandt WJ, Liu Y, Heise CE, Hoyte K, Luk W, Lu Y, Peng K, Wu P, Rouge L, Zhang Y, Lazarus RA, Scearce-Levie K, Wang W, Wu Y, Tessier-Lavigne M, Watts RJ.
  Sci Transl Med. 2011;3(84):84ra43.
  Publication year: 2011

  Amyloid β1-40 assay

  Streptavidin & affinity reagents toolbox

  
• Identification of inhibitors for putative malaria drug targets among novel antimalarial compounds.
  Crowther GJ, Napuli AJ, Gilligan JH, Gagaring K, Borboa R, Francek C, Chen Z, Dagostino EF, Stockmyer JB, Wang Y, Rodenbough PP, Castaneda LJ, Leibly DJ, Bhandari J, Gelb MH, Brinker A, Engels IH, Taylor J, Chatterjee AK, Fantauzzi P, Glynne RJ, Van Voorhis WC, Kuhen KL.
  Mol Biochem Parasitol. 2011;175(1):21-9.
  Publication year: 2011

  HTRF® Transcreener® ADP
• Development of a Cyclic Adenosine Monophosphate Assay for G(i)-Coupled G Protein-Coupled Receptors by Utilizing the Endogenous Calcitonin Activity in Chinese Hamster Ovary Cells.
  Wang Y, Kong Y, Shei GJ, Kang L, Cvijic ME
  Assay Drug Dev Technol. 2011;9(5):522-31.
  Publication year: 2011

  cAMP cell-based assay

  
• In vitro modulation of LPS/calcimycin induced inflammatory and allergic mediators by pure compounds of Andrographis paniculata (King of bitters) extract.
  Chandrasekaran CV, Thiyagarajan P, Deepak HB, Agarwal A.
  Int Immunopharmacol. 2011;11(1):79-84.
  Publication year: 2011

  Histamine assay

  Prostaglandin E2 assay

  Leukotriene B4 & Leukotriene C4 assays
• Trans-activation between 7TM domains: implication in heterodimeric GABAB receptor activation.
  Monnier C, Tu H, Bourrier E, Vol C, Lamarque L, Trinquet E, Pin JP, Rondard P.
  EMBO J. 2011;30(1):32-42.
  Publication year: 2011

  IP-One HTRF® assay

  Tag-lite® toolbox
• Anti-diabetic and anti-adipogenic effects of a novel selective 11β-hydroxysteroid dehydrogenase type 1 inhibitor, KR-66344.
  Park JS, Rhee SD, Kang NS, Jung WH, Kim HY, Kim JH, Kang SK, Cheon HG, Ahn JH, Kim KY.
  Biochem Pharmacol. 2011;81(8):1028-35
  Publication year: 2011

  Cortisol assay
• Microtiter plate quantification of mutant and wild-type huntingtin normalized to cell count.
  Weiss A, Grueninger S, Abramowski D, Giorgio FP, Lopatin MM, Rosas HD, Hersch S, Paganetti P.
  Anal Biochem. 2011;410(2):304-6.
  Publication year: 2011

  Streptavidin & affinity reagents toolbox

  Biochemical and cell-based assay development
• Engineering a high-affinity anti-IL-15 antibody: crystal structure reveals an alpha-helix in VH CDR3 as key component of paratope.
  Lowe DC, Gerhardt S, Ward A, Hargreaves D, Anderson M, Ferraro F, Pauptit RA, Pattison DV, Buchanan C, Popovic B, Finch DK, Wilkinson T, Sleeman M, Vaughan TJ, Mallinder PR
  J Mol Biol. 2011;406(1):160-75.
  Publication year: 2011

  Streptavidin & affinity reagents toolbox

  Biochemical targets
• Time-resolved Fluorescence Resonance Energy Transfer (TR-FRET) to Analyze the Disruption of EGFR/HER2 Dimers: A new method to evaluate the efficiency of targeted therapy using monoclonal antibodies.
  Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardès T, Poul MA, Mathis G, Bazin H, Pèlegrin A.
  J Biol Chem. 2011;286(13):11337-45.
  Publication year: 2011

  Toolbox products and services

  HTRF® Services

  Antibody screening on cell surface targets
• Synthesis and 11β hydroxysteroid dehydrogenase 1 inhibition of thiazolidine derivatives with an adamantyl group.
  Kwon SW, Kang SK, Lee JH, Bok JH, Kim CH, Dal Rhee S, Jung WH, Kim HY, Bae MA, Song JS, Ha DC, Cheon HG, Kim KY, Ahn JH.
  Bioorg Med Chem Lett. 2011;21(1):435-9.
  Publication year: 2011

  Cortisol assay
• Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a.
  Leyris JP, Roux T, Trinquet E, Verdié P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M'kadmi C, Martinez J, Mary S, Banères JL, Marie J.
  Anal Biochem. 2011;408(2):253-62.
  Publication year: 2011

  Tag-lite® binding assays

  Ghrelin GHSR1A receptor ligand binding assay

  
• A new approach to analyze cell surface protein complexes reveals specific heterodimeric metabotropic glutamate receptors.
  Doumazane E, Scholler P, Zwier JM, Eric T, Rondard P, Pin JP.
  FASEB J. 2011;25(1):66-77.
  Publication year: 2011

  IP-One HTRF® assay
• Designed inhibitors of insulin-degrading enzyme regulate the catabolism and activity of insulin.
  Leissring MA, Malito E, Hedouin S, Reinstatler L, Sahara T, Abdul-Hay SO, Choudhry S, Maharvi GM, Fauq AH, Huzarska M, May PS, Choi S, Logan TP, Turk BE, Cantley LC, Manolopoulou M, Tang WJ, Stein RL, Cuny GD, Selkoe DJ.
  PLoS One. 2010 May 7;5(5):e10504.
  Publication year: 2010

  Insulin assay
• Quercetin potentiates insulin secretion and protects INS-1 pancreatic β-cells against oxidative damage via the ERK1/2 pathway
  Youl E, Bardy G, Magous R, Cros G, Sejalon F, Virsolvy A, Richard S, Quignard JF, Gross R, Petit P, Bataille D, Oiry C
  Br J Pharmacol. 2010;161(4):799-814.
  Publication year: 2010

  Insulin assay
• KR-003048, a potent, orally active inhibitor of p38 mitogen-activated protein kinase.
  Montalban AG, Boman E, Chang CD, Ceide SC, Dahl R, Dalesandro D, Delaet NG, Erb E, Ernst J, Gibbs A, Kahl J, Kessler L, Lundström J, Miller S, Nakanishi H, Roberts E, Saiah E, Sullivan R, Wang Z, Larson CJ
  Eur J Pharmacol. 2010 Apr 25;632(1-3):93-102.
  Publication year: 2010

  Human & Mouse TNFα assays
• The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals
  Martín R, Durroux T, Ciruela F, Torres M, Pin JP, Sánchez-Prieto J.
  J Biol Chem. 2010;285(23):17907-17.
  Publication year: 2010

  cAMP cell-based assay

  IP-One HTRF® assay
• Establishing and functional characterization of an HEK-293 cell line expressing autofluorescently tagged beta-actin (pEYFP-ACTIN) and the neurokinin type 1 receptor (NK1-R).
  Hrovat A, Zavec AB, Pogacnik A, Frangez R, Vrecl M.
  Cell Mol Biol Lett. 2010;15(1):55-69.
  Publication year: 2010

  IP-One ELISA assay
• The Gq/G11-mediated signaling pathway is critical for autocrine potentiation of insulin secretion in mice.
  Sassmann A, Gier B, Grone HJ, Drews G, Offermanns S, Wettschureck N.
  J Clin Invest. 2010;120(6):2184-93.
  Publication year: 2010

  IP-One ELISA assay
• Identification and analysis of two splice variants of human G2A generated by alternative splicing.
  Ogawa A, Obinata H, Hattori T, Kishi M, Tatei K, Ishikawa O, Izumi T.
  J Pharmacol Exp Ther. 2010;332(2):469-78.
  Publication year: 2010

  IP-One ELISA assay
• Odorant-stimulated phosphoinositide signaling in mammalian olfactory receptor neurons.
  Klasen K, Corey EA, Kuck F, Wetzel CH, Hatt H, Ache BW.
  Cell Signal. 2010;22(1):150-7.
  Publication year: 2010

  IP-One ELISA assay
• Differential association modes of the thrombin receptor PAR1 with Galphai1, Galpha12, and beta-arrestin 1.
  Ayoub MA, Trinquet E, Pfleger KD, Pin JP
  FASEB J. 2010;24(9):3522-35.
  Publication year: 2010

  HTRF® Anti-tag reagents toolbox
• Neuroprotection by selective allosteric potentiators of the EP2 prostaglandin receptor.
  Jiang J, Ganesh T, Du Y, Thepchatri P, Rojas A, Lewis I, Kurtkaya S, Li L, Qui M, Serrano G, Shaw R, Sun A, Dingledine R
  Proc Natl Acad Sci U S A. 2010;107(5):2307-12.
  Publication year: 2010

  cAMP cell-based assay
• A uHTS approach for the identification of beta-adrenoceptor ligands.
  Tasler S, Baumgartner R, Aschenbrenner A, Ammendola A, Wolf K, Wieber T, Schachtner J, Blisse M, Quotschalla U, Ney P
  Bioorg Med Chem Lett. 2010;20(11):3399-404.
  Publication year: 2010

  cAMP cell-based assay
• Application of fragment-based NMR screening, X-ray crystallography, structure-based design, and focused chemical library design to identify novel microM leads for the development of nM BACE-1 (beta-site APP cleaving enzyme 1) inhibitors.
  Wang YS, Strickland C, Voigt JH, Kennedy ME, Beyer BM, Senior MM, Smith EM, Nechuta TL, Madison VS, Czarniecki M, McKittrick BA, Stamford AW, Parker EM, Hunter JC, Greenlee WJ, Wyss DF.
  J Med Chem. 2010;53(3):942-50.
  Publication year: 2010

   
• Discovery of aminopyridines substituted with benzoxazole as orally active c-Met kinase inhibitors.
  Cho SY, Han SY, Ha JD, Ryu JW, Lee CO, Jung H, Kang NS, Kim HR, Koh JS, Lee J.
  Bioorg Med Chem Lett. 2010;20(14):4223-7
  Publication year: 2010

  HTRF® KinEASE™
• A homogeneous time-resolved fluorescence-based high-throughput screening system for discovery of inhibitors of IKKbeta-NEMO interaction.
  Gotoh Y, Nagata H, Kase H, Shimonishi M, Ido M.
  Anal Biochem. 2010;405(1):19-27.
  Publication year: 2010

  HTRF® Anti-tag reagents toolbox

  
• SNS-314, a pan-Aurora kinase inhibitor, shows potent anti-tumor activity and dosing flexibility in vivo.
  Arbitrario JP, Belmont BJ, Evanchik MJ, Flanagan WM, Fucini RV, Hansen SK, Harris SO, Hashash A, Hoch U, Hogan JN, Howlett AR, Jacobs JW,
  Cancer Chemother Pharmacol. 2010;65(4):707-17.
  Publication year: 2010

  Kinase toolbox reagents
• A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization.
  Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.
  Assay Drug Dev Technol. 2010;8(1):96-105.
  Publication year: 2010

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox
• Discovery of cyclicsulfonamide derivatives as 11 beta-hydroxysteroid dehydrogenase 1 inhibitors.
  Kim SH, Ramu R, Kwon SW, Lee SH, Kim CH, Kang SK, Rhee SD, Bae MA, Ahn SH, Ha DC, Cheon HG, Kim KY, Ahn JH.
  Bioorg Med Chem Lett. 2010;20(3):1065-9.
  Publication year: 2010

  Cortisol assay
• A fluorescent ligand-binding alternative using Tag-lite® technology.
  Zwier JM, Roux T, Cottet M, Durroux T, Douzon S, Bdioui S, Gregor N, Bourrier E, Oueslati N, Nicolas L, Tinel N, Boisseau C, Yverneau P, Charrier-Savournin F, Fink M, Trinquet E.
  J Biomol Screen. 2010;15(10):1248-59.
  Publication year: 2010

  Tag-lite® binding assays

  Cholecystokinin CCK1 receptor ligand binding assay

  Cholecystokinin CCK2 receptor ligand binding assay

  Chemokine CXCR4 receptor ligand binding assay

  
• GalR2-positive allosteric modulator exhibits anticonvulsant effects in animal models.
  Lu X, Roberts E, Xia F, Sanchez-Alavez M, Liu T, Baldwin R, Wu S, Chang J, Wasterlain CG, Bartfai T
  Proc Natl Acad Sci U S A. 2010;107(34):15229-34.
  Publication year: 2010

  cAMP cell-based assay

  IP-One HTRF® assay
• Subtlety of the structure-affinity and structure-efficacy relationships around a nonpeptide oxytocin receptor agonist.
  Frantz MC, Rodrigo J, Boudier L, Durroux T, Mouillac B, Hibert M.
  J Med Chem. 2010 Feb 25;53(4):1546-62.
  Publication year: 2010

  cAMP cell-based assay

  IP-One HTRF® assay
• Functional crosstalk between GPCRs: with or without oligomerization.
  Prezeau L, Rives ML, Comps-Agrar L, Maurel D, Kniazeff J, Pin JP.
  Curr Opin Pharmacol. 2010;10(1):6-13.
  Publication year: 2010

  HTRF® Anti-tag reagents toolbox

  Tag-lite® toolbox
• A high-throughput small-molecule ligand screen targeted to agonists and antagonists of the G-protein-coupled receptor GPR54.
  Kuohung W, Burnett M, Mukhtyar D, Schuman E, Ni J, Crowley WF, Glicksman MA, Kaiser UB.
  J Biomol Screen. 2010;15(5):508-17.
  Publication year: 2010

  IP-One HTRF® assay
• Development of an improved IP(1) assay for the characterization of 5-HT(2C) receptor ligands
  Zhang JY, Kowal DM, Nawoschik SP, Dunlop J, Pausch MH, Peri R.
  Assay Drug Dev Technol. 2010;8(1):106-13.
  Publication year: 2010

  IP-One HTRF® assay
• Evaluation of nonradioactive cell-free cAMP assays for measuring in vitro phosphodiesterase activity
  Eapen MS, Sodhi R, Balakrishnan G, Dastidar S, Ray A, Vijayakrishnan L
  Pharmacology. 2010;85(5):280-285.
  Publication year: 2010

  cAMP cell-based assay

  
• Biphasic suppression of appetite by cannabinoid CB1 receptor antagonists with distinct functional activities
  Lin Y, Shia KS, Hsiao WC, Hsieh WP, Yeh TK, Tseng SL, Hsu CY, Chao YS, Hung MS
  Pharmacol Res. 2010;62(4):337-43.
  Publication year: 2010

  cAMP cell-based assay
• Identification and characterization of a small molecule antagonist of human VPAC(2) receptor
  Chu A, Caldwell JS, Chen YA
  Mol Pharmacol. 2010;77(1):95-101.
  Publication year: 2010

  cAMP cell-based assay
• Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET)...
  Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
  J Biol Chem. 2010;285(30):23318-30
  Publication year: 2010

  Tag-lite® toolbox

  
• Time-resolved FRET between GPCR ligands reveals oligomers in native tissues.
  Albizu L, Cottet M, Kralikova M, Stoev S, Seyer R, Brabet I, Roux T, Bazin H, Bourrier E, Lamarque L, Breton C, Rives ML, Newman A, Javitch J, Trinquet E, Manning M, Pin JP, Mouillac B, Durroux T.
  Nat Chem Biol. 2010;6(8):587-94.
  Publication year: 2010

  Tag-lite® toolbox

  Tag-lite® binding assays

  Vasopressin V1A receptor ligand binding assay

  
• Suppression of guanylyl cyclase (beta1 subunit) expression impairs neurite outgrowth and synapse maturation in cultured cerebellar granule cells.
  López-Jiménez ME, Bartolomé-Martín D, Sánchez-Prieto J, Torres M.
  Cell Death Differ. 2009 Sep;16(9):1266-78.
  Publication year: 2009

  cGMP assay
• Loss of Lkb1 in adult beta cells increases beta cell mass and enhances glucose tolerance in mice
  Fu A, Ng AC, Depatie C, Wijesekara N, He Y, Wang GS, Bardeesy N, Scott FW, Touyz RM, Wheeler MB, Screaton RA.
  Cell Metab. 2009 Oct;10(4):285-95.
  Publication year: 2009

  Insulin assay
• The E3 ubiquitin-ligase Bmi1/Ring1A controls the proteasomal degradation of Top2alpha cleavage complex - a potentially new drug target
  Alchanati I, Teicher C, Cohen G, Shemesh V, Barr HM, Nakache P, Ben-Avraham D, Idelevich A, Angel I, Livnah N, Tuvia S, Reiss Y, Taglicht D, Erez O.
  PLoS One. 2009;4(12):e8104.
  Publication year: 2009

  HTRF® Anti-tag reagents toolbox
• Inhibition of heterotrimeric G protein signaling by a small molecule acting on Galpha subunit.
  Ayoub MA, Damian M, Gespach C, Ferrandis E, Lavergne O, De Wever O, Baneres JL, Pin JP, Prevost GP
  J Biol Chem. 2009;284(42):29136-45.
  Publication year: 2009

  cAMP cell-based assay

  IP-One HTRF® assay
• Discovery and structure-activity relationships of pentanedioic acid diamides as potent inhibitors of 11beta-hydroxysteroid dehydrogenase type I.
  Roche D, Carniato D, Leriche C, Lepifre F, Christmann-Franck S, Graedler U, Charon C, Bozec S, Doare L, Schmidlin F, Lecomte M, Valeur E.
  Bioorg Med Chem Lett. 2009;19(10):2674-8.
  Publication year: 2009

  Cortisol assay
• A miniaturized 1536-well format gamma-secretase assay.
  Shelton CC, Tian Y, Shum D, Radu C, Djaballah H, Li YM.
  Assay Drug Dev Technol. 2009;7(5):461-70.
  Publication year: 2009

  Streptavidin & affinity reagents toolbox

  Custom labeling
• Fully human monoclonal antibodies antagonizing the glucagon receptor improve glucose homeostasis in mice and monkeys
  Yan H, Gu W, Yang J, Bi V, Shen Y, Lee E, Winters KA, Komorowski R, Zhang C, Patel JJ, Caughey D, Elliott GS, Lau YY, Wang J, Li YS, Boone T, Lindberg RA, Hu S, Véniant MM.
  J Pharmacol Exp Ther. 2009;329(1):102-11.
  Publication year: 2009

  cAMP cell-based assay

  Antibody mechanism of action study

  
• Non-redundant roles of phosphoinositide 3-kinase isoforms alpha and beta in glycoprotein VI-induced platelet signaling and thrombus formation.
  Gilio K, Munnix IC, Mangin P, Cosemans JM, Feijge MA, van der Meijden PE, Olieslagers S, Chrzanowska-Wodnicka MB, Lillian R, Schoenwaelder S, Koyasu S, Sage SO, Jackson SP, Heemskerk JW.
  J Biol Chem. 2009;284(49):33750-62.
  Publication year: 2009

  IP-One ELISA assay
• Cav1.2 and Cav1.3 are differentially coupled to glucagon-like peptide-1 potentiation of glucose-stimulated insulin secretion in the pancreatic beta-cell line INS-1.
  Jacobo SM, Guerra ML, Hockerman GH.
  J Pharmacol Exp Ther. 2009;331(2):724-32.
  Publication year: 2009

  IP-One ELISA assay
• Distinct role of PLCbeta3 in VEGF-mediated directional migration and vascular sprouting.
  Bhattacharya R, Kwon J, Li X, Wang E, Patra S, Bida JP, Bajzer Z, Claesson-Welsh L, Mukhopadhyay D.
  J Cell Sci. 2009;122(Pt 7):1025-34.
  Publication year: 2009

  IP-One ELISA assay
• Development of a fluorescent-tagged kinase assay system for the detection and characterization of allosteric kinase inhibitors.
  Simard JR, Getlik M, Grutter C, Pawar V, Wulfert S, Rabiller M, Rauh D.
  J Am Chem Soc. 2009;131(37):13286-96.
  Publication year: 2009

  Kinase toolbox reagents

  HTRF® Anti-tag reagents toolbox
• High-throughput screening to identify inhibitors which stabilize inactive kinase conformations in p38alpha.
  Simard JR, Grutter C, Pawar V, Aust B, Wolf A, Rabiller M, Wulfert S, Robubi A, Kluter S, Ottmann C, Rauh D.
  J Am Chem Soc. 2009;131(51):18478-88.
  Publication year: 2009

  Kinase toolbox reagents

  HTRF® Anti-tag reagents toolbox
• Affinity maturation of a humanized rat antibody for anti-RAGE therapy.
  Finlay WJ, Cunningham O.
  J Mol Biol. 2009;388(3):541-58.
  Publication year: 2009

  Streptavidin & affinity reagents toolbox

  Biochemical targets

  HTRF® Dye labeling kits
• E6201 [(3S,4R,5Z,8S,9S,11E)-14-(ethylamino)-8, 9,16-trihydroxy-3,4-dimethyl-3,4,9,19-tetrahydro-1H-2-benzoxacyclotetradecine-1,7(8H)-dione], a novel kinase inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase-1 and mi
  Goto M, Chow J, Muramoto K, Chiba K, Yamamoto S, Fujita M, Obaishi H, Tai K, Mizui Y, Tanaka I, Young D, Yang H, Wang YJ, Shirota H, Gusovsky F.
  J Pharmacol Exp Ther. 2009;331(2):485-95.
  Publication year: 2009

  Kinase toolbox reagents
• Cell-based assay for screening 11beta-hydroxysteroid dehydrogenase 1 inhibitors.
  Cho YS, Kim CH, Cheon HG
  Anal Biochem. 2009;392(2):110-6.
  Publication year: 2009

  Cortisol assay
• Crosstalk between GABA(B) and mGlu1a receptors reveals new insight into GPCR signal integration.
  Rives ML, Vol C, Fukazawa Y, Tinel N, Trinquet E, Ayoub MA, Shigemoto R, Pin JP, Prézeau L.
  EMBO J. 2009;28(15):2195-208.
  Publication year: 2009

  HTRF® Anti-tag reagents toolbox

  Tag-lite® toolbox
• Activating autoantibodies against the calcium-sensing receptor detected in two patients with autoimmune polyendocrine syndrome type 1
  Kemp EH, Gavalas NG, Krohn KJ, Brown EM, Watson PF, Weetman AP.
  J Clin Endocrinol Metab. 2009;94(12):4749-56.
  Publication year: 2009

  IP-One ELISA assay
• A homogeneous resonance energy transfer-based assay to monitor MutS/DNA interactions.
  Lopez-Crapez E, Malinge JM, Gatchitch F, Casano L, Langlois T, Pugniere M, Roquet F, Mathis G, Bazin H.
  Anal Biochem. 2008;383(2):301-6.
  Publication year: 2008

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox
• The signalling profile of recombinant human orexin-2 receptor.
  Tang J, Chen J, Ramanjaneya M, Punn A, Conner AC, Randeva HS.
  Cell Signal. 2008;20(9):1651-61.
  Publication year: 2008

  IP-One ELISA assay
• TRP-independent inhibition of the phospholipase C pathway by natural sensory ligands.
  Kim KY, Bang S, Han S, Nguyen YH, Kang TM, Kang KW, Hwang SW.
  Biochem Biophys Res Commun. 2008;370(2):295-300.
  Publication year: 2008

  IP-One ELISA assay
• Histamine suppresses fibulin-5 and insulin-like growth factor-II receptor expression in melanoma.
  Pos Z, Wiener Z, Pocza P, Racz M, Toth S, Darvas Z, Molnar V, Hegyesi H, Falus A.
  Cancer Res. 2008;68(6):1997-2005.
  Publication year: 2008

  IP-One ELISA assay
• A novel method for determination of the affinity of protein: protein interactions in homogeneous assays.
  Newton P, Harrison P, Clulow S.
  J Biomol Screen. 2008;13(7):674-82.
  Publication year: 2008

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox
• Structural determination of estrogen-related receptor gamma in the presence of phenol derivative compounds.
  Abad MC, Askari H, O'Neill J, Klinger AL, Milligan C, Lewandowski F, Springer B, Spurlino J, Rentzeperis D.
  J Steroid Biochem Mol Biol. 2008;108(1-2):44-54.
  Publication year: 2008

  HTRF® Anti-tag reagents toolbox
• HTRF-based assay for microsomal prostaglandin E2 synthase-1 activity.
  Goedken ER, Gagnon AI, Overmeyer GT, Liu J, Petrillo RA, Burchat AF, Tomlinson MJ.
  J Biomol Screen. 2008;13(7):619-25.
  Publication year: 2008

  Prostaglandin E2 assay
• Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies: application to GPCR oligomerization
  Maurel D, Comps-Agrar L, Brock C, Rives ML, Bourrier E, Ayoub MA, Bazin H, Tinel N, Durroux T, Prezeau L, Trinquet E, Pin JP.
  Nat Methods. 2008;5(6):561-7.
  Publication year: 2008

  HTRF® Anti-tag reagents toolbox

  Tag-lite® toolbox

  
• Evaluating the utility of HTRF Transcreener ADP assay technology: A comparison with the standard HTRF assay technology
  Hong L, Quinn CM, Jia Y.
  Anal Biochem. 2009;391(1):31-8
  Publication year: 2008

  HTRF® Transcreener® ADP

  Kinase toolbox reagents
• Comparison on Functional Assays for Gq-Coupled GPCRs by Measuring Inositol Monophospate-1 and Intracellular Calcium in 1536-Well Plate Format
  Liu K, Titus S, Southall N, Zhu P, Inglese J, Austin CP, Zheng W.
  Curr Chem Genomics. 2008;1:70-8.
  Publication year: 2008

  IP-One HTRF® assay
• Development of a HTRF Kinase Assay for Determination of Syk Activity
  Harbert C, Marshall J, Soh S, Steger K
  Curr Chem Genomics. 2008;1:20-6.
  Publication year: 2008

  Kinase toolbox reagents

  HTRF® KinEASE™
• A One-Day, Dispense-Only IP-One HTRF Assay for High-Throughput Screening of Gq Protein-Coupled Receptors: Towards Cells as Reagents
  Bergsdorf C, Kropp-Goerkis C, Kaehler I, Ketscher L, Boemer U, Parczyk K, Bader B.
  Assay Drug Dev Technol. 2008;6(1):39-53
  Publication year: 2008

  IP-One HTRF® assay
• Thrombocytes respond to lipopolysaccharide through Toll-like receptor-4, and MAP kinase and NF-[kappa]B pathways leading to expression of interleukin-6 and cyclooxygenase-2 with production of PGE2
  Scott T, Owens MD.
  Mol Immunol. 2008;45(4):1001-8
  Publication year: 2008

  Prostaglandin E2 assay
• PDE5 inhibitors: An original access to novel potent arylated analogues of tadalafil
  Beghyn T, Hounsou C, Deprez BP.
  Bioorg Med Chem Lett. 2007;17(3):789-92
  Publication year: 2007

  cGMP assay
• The ubiquitin E3 ligase POSH regulates calcium homeostasis through spatial control of Herp
  Tuvia S, Taglicht D, Erez O, Alroy I, Alchanati I, Bicoviski V, Dori-Bachash M, Ben-Avraham D, Reiss Y.
  J Cell Biol. 2007;177(1):51-61
  Publication year: 2007

  Ubiquitin assay toolbox

  HTRF® Anti-tag reagents toolbox

  Custom labeling
• Development of a homogeneous time-resolved fluorescence leukotriene B4 assay for determining the activity of leukotriene A4 hydrolase
  Liang AM, Claret E, Ouled-Diaf J, Jean A, Vogel D, Light DR, Jones SW, Guilford WJ, Parkinson JF, Snider RM.
  J Biomol Screen. 2007;12(4):536-45.
  Publication year: 2007

  Leukotriene B4 & Leukotriene C4 assays
• Toward efficient drug screening by homogeneous assays based on the development of new fluorescent vasopressin and oxytocin receptor ligands.
  Albizu L, Teppaz G, Seyer R, Bazin H, Ansanay H, Manning M, Mouillac B, Durroux T.
  J Med Chem. 2007;50(20):4976-85.
  Publication year: 2007

  IP-One ELISA assay

  Tag-lite® binding assays

  Vasopressin V1A receptor ligand binding assay

  Vasopressin V2 receptor ligand binding assay

  
• Specific Leukotriene Receptors Couple to Distinct G Proteins to Effect Stimulation of Alveolar Macrophage Host Defense Functions
  Peres CM, Aronoff DM, Serezani CH, Flamand N, Faccioli L, Peters-Golden M
  J. Immunol., Oct 2007; 179: 5454 - 5461.
  Publication year: 2007

  IP-One ELISA assay
• Anorexia induced by activation of serotonin 5-HT4 receptors is mediated by increases in CART in the nucleus accumbens
  Jean A, Conductier G, Manrique C, Bouras C, Berta P, Hen R, Charnay Y, Bockaert J, Compan V
  Proc Natl Acad Sci U S A. 2007;104(41):16335-40
  Publication year: 2007

  cAMP cell-based assay

  
• Real-Time Analysis of Agonist-Induced Activation of Protease-Activated Receptor 1/Galpha i1 Protein Complex Measured by Bioluminescence Resonance Energy Transfer in Living Cells
  Ayoub MA, Maurel D, Binet V, Fink M, Prézeau L, Ansanay H, Pin JP.
  Mol Pharmacol. 2007;71(5):1329-40
  Publication year: 2007

  cAMP cell-based assay

  IP-One HTRF® assay
• Identifying Nonselective Hits from a Homogeneous Calcium Assay Screen
  Cassutt KJ, Orsini MJ, Abousleiman M, Colone D, Tang W.
  J Biomol Screen. 2007;12(2):285-7.
  Publication year: 2007

  IP-One HTRF® assay
• Identifying Nonselective Hits from a Homogeneous Calcium Assay Screen
  Cassutt KJ, Orsini MJ, Abousleiman M, Colone D, Tang W
  J Biomol Screen. 2007;12(2):285-7.
  Publication year: 2007

  IP-One HTRF® assay
• Identification of ligands with bicyclic scaffolds provides insights into mechanisms of estrogen receptor subtype selectivity.
  Hsieh RW, Rajan SS, Sharma SK, Guo Y, DeSombre ER, Mrksich M, Greene GL.
  J Biol Chem. 2006;281(26):17909-19.
  Publication year: 2006

  HTRF® Anti-tag reagents toolbox

  
• Cellular Ser/Thr-Kinase Assays Using Generic Peptide Substrates
  Adams DG, Wang Y, Mak PA, Chyba J, Shalizi O, Matzen J, Anderson P, Smith TR, Garcia M, Welch GL, Claret EJ, Fink M, Orth AP, Caldwell JS, Brinker A.
  Curr Chem Genomics. 2008;1:54-64.
  Publication year: 2006

  Kinase toolbox reagents

  HTRF® Services
• D-myo-Inositol 1-phosphate as a surrogate of D-myo-inositol 1,4,5-tris phosphate to monitor G protein-coupled receptor activation
  Trinquet E, Fink M, Bazin H, Grillet F, Maurin F, Bourrier E, Ansanay H, Leroy C, Michaud A, Durroux T, Maurel D, Malhaire F, Goudet C, Pin JP, Naval M, Hernout O, Chrétien F, Chapleur Y, Mathis G.
  Anal Biochem. 2006;358(1):126-35.
  Publication year: 2006

  IP-One HTRF® assay
• Comparative analysis of various in vitro COT kinase assay formats and their applications in inhibitor identification and characterization
  Jia Y, Quinn CM, Clabbers A, Talanian R, Xu Y, Wishart N, Allen H.
  Anal Biochem. 2006;350(2):268-76.
  Publication year: 2006

  Kinase toolbox reagents

  
• Homogeneous time-resolved fluorescence and its applications for kinase assays in drug discovery.
  Jia Y, Quinn CM, Gagnon AI, Talanian R.
  Anal Biochem. 2006 Sep 15;356(2):273-81.
  Publication year: 2006

  Kinase toolbox reagents

  
• Lysophosphatidylcholine enhances glucose-dependent insulin secretion via an orphan G-protein-coupled receptor.
  Soga T, Ohishi T, Matsui T, Saito T, Matsumoto M, Takasaki J, Matsumoto S, Kamohara M, Hiyama H, Yoshida S, Momose K, Ueda Y, Matsushime H, Kobori M, Furuichi K
  Biochem Biophys Res Commun. 2005;326(4):744-51.
  Publication year: 2005

  cAMP cell-based assay
• Evidence for a single heptahelical domain being turned on upon activation of a dimeric GPCR
  Hlavackova V, Goudet C, Kniazeff J, Zikova A, Maurel D, Vol C, Trojanova J, Prézeau L, Pin JP, Blahos J.
  EMBO J. 2005;24(3):499-509.
  Publication year: 2005

  HTRF® Anti-tag reagents toolbox

  
• Purification and kinetic characterization of recombinant human mitogen-activated protein kinase kinase kinase COT and the complexes with its cellular partner NF-kB1 p105
  Jia Y, Quinn CM, Bump NJ, Clark KM, Clabbers A, Hardman J, Gagnon A, Kamens J, Tomlinson MJ, Wishart N, Allen H.
  Arch Biochem Biophys. 2005;441(1):64-74.
  Publication year: 2005

  Kinase toolbox reagents
• Suppression of CD4+ T cell activation by a novel inhibitor of Src family kinases
  McRae BL, Wallace C, Dixon KF, Roux A, Mohan S, Jia Y, Presky DH, Tracey DE, Hirst GC.
  Int Immunopharmacol. 2005;5(4):667-77.
  Publication year: 2005

  Kinase toolbox reagents
• A Separation-Free Assay for the Detection of Mutations: Combination of Homogeneous Time-Resolved Fluorescence and Minisequencing
  Lopez-Crapez E, Bazin H, Chevalier J, Trinquet E, Grenier J, Mathis G.
  Methods. 2005; 25: 468-475
  Publication year: 2005

  HTRF® Labeling reagents toolbox

  Streptavidin & affinity reagents toolbox
• Cell surface detection of membrane protein interaction with homogeneous time-resolved fluorescence resonance energy transfer technology
  Maurel D, Kniazeff J, Mathis G, Trinquet E, Pin JP, Ansanay H.
  Anal Biochem. 2004;329(2):253-62.
  Publication year: 2004

  HTRF® Anti-tag reagents toolbox
• Molecular determinants involved in the allosteric control of agonist affinity in GABAB receptor by the GABAB2 subunit
  Liu J, Maurel D, Etzol S, Brabet I, Ansanay H, Pin JP, Rondard P.
  J Biol Chem. 2004;279(16):15824-30.
  Publication year: 2004

  HTRF® Anti-tag reagents toolbox
• Closed state of both binding domains of homodimeric mGlu receptors is required for full activity
  Kniazeff J, Bessis A-S, Maurel D, Ansanay H, Prezeau L and Pin J-P
  Nat Struct Mol Biol. 2004;11(8):706-13.
  Publication year: 2004

  HTRF® Anti-tag reagents toolbox

  
• A homogeneous time-resolved fluorescence detection of telomerase activity.
  Gabourdes M, Bourguine V, Mathis G, Bazin H, Alpha-Bazin B
  Anal Biochem. 2004;333(1):105-13
  Publication year: 2004

  HTRF® Labeling reagents toolbox
• Homogeneous Time resolved Fluorescence Assay to Measure Histamine Release
  Claret E, Ouled-Diaf J, Seguin P
  Comb Chem High Throughput Screen. 2003 Dec;6(8):789-94.
  Publication year: 2003

  Histamine assay
• High throughput screening technologies for direct cyclic AMP measurement.
  Gabriel D, Vernier M, Pfeifer MJ, Dasen B, Tenaillon L, Bouhelal R
  Assay Drug Dev Technol. 2003;1(2):291-303.
  Publication year: 2003

  cAMP cell-based assay
• Homogeneous assays allow direct in well cytokine level quantification.
  Achard S, Jean A, Lorphelin D, Amoravain M, Claret EJ.
  Assay Drug Dev Technol. 2003;1(1 Pt 2):181-5.
  Publication year: 2003

  Human & Mouse TNFα assays

  Human & Mouse IFNγ assays

  Human IL1β assay

  Human & Mouse IL6 assays
• Measuring human beta-secretase (BACE1) activity using homogeneous time-resolved fluorescence
  Kennedy ME,Wang W, Song L, Lee J, Zhang L, Wong G, Wan L, Parker E
  Anal. Biochem. 2003; 319:49-55
  Publication year: 2003

   
• Miniaturizable homogeneous time-resolved fluorescence assay for carboxypeptidase B activity.
  Ferrer M, Zuck P, Kolodin G, Mao SS, Peltier RR, Bailey C, Gardell SJ, Strulovici B, Inglese J.
  Anal Biochem. 2003;317(1):94-8.
  Publication year: 2003

  Streptavidin & affinity reagents toolbox

  Biochemical and cell-based assay development

  
• High-throughput methods to detect dimerization of Bcl-2 family proteins
  Whitfield J, Harada K, Bardelle C, Staddon JM.
  Anal Biochem. 2003;322(2):170-8
  Publication year: 2003

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox

  
• Homogeneous phosphodiesterase and hybridization assays using Europium Cryptate: Oligonucleotide conjugates.
  Bazin H, Guillemer S, Mathis G.
  J Fluorescence. 2002;12(2):245-8
  Publication year: 2002

  HTRF® Labeling reagents toolbox
• Homogeneous time-resolved fluorescence assay for identifying p53 interactions with its protein partners, directly in a cellular extract
  Leblanc V, Delaunay V, Claude Lelong J, Gas F, Mathis G, Grassi J, May E.
  Anal Biochem. 2002;308(2):247-54.
  Publication year: 2002

  HTRF® Labeling reagents toolbox
• Time-resolved amplification of cryptate emission: a versatile technology to trace biomolecular interactions
  Bazin H, Trinquet E, Mathis G
  J. biotechnol. 2002; 82: 233-50.
  Publication year: 2002

  Ubiquitin assay toolbox

  Streptavidin & affinity reagents toolbox
• A homogeneous caspase 3 activity assay using HTRF® technology.
  Préaudat M, Ouled-Diaf J, Alpha-Bazin B, Mathis G, Mitsugi T, Aono Y, Takahashi K, Takemoto H.
  J Biomol Screen. 2002;7(3):267-74.
  Publication year: 2002

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox
• Homogeneous time-resolved fluorescence resonance energy transfer using rare earth cryptates as a tool for probing molecular interactions in biology.
  Bazin H, Preaudat M, Trinquet E, Mathis G
  Spectrochimica Acta (A). 2001;57:2197-211
  Publication year: 2001

  HTRF® chemistry
• Use of homogeneous time-resolved fluorescence energy transfer in the measurement of nuclear receptor activation.
  Zhou G, Cummings R, Hermes J, Moller DE.
  Methods. 2001;25(1):54-61.
  Publication year: 2001

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox

  
• A homogeneous europium cryptate-based assay for the diagnosis of mutations by time-resolved fluorescence resonance energy transfer.
  Lopez-Crapez E, Bazin H, Andre E, Noletti J, Grenier J, Mathis G.
  Nucleic Acids Res. 2001;29: 14e70.
  Publication year: 2001

  HTRF® Labeling reagents toolbox

  Streptavidin & affinity reagents toolbox
• Allophycocyanin 1 as a near-infrared fluorescent tracer : isolation, characterization, chemical modification, and use in homogeneous fluorescence resonance energy transfer.
  Trinquet E, Maurin F, Preaudat M, Mathis G
  Anal Biochem. 2001;296:232-44
  Publication year: 2001

  HTRF® chemistry
• High throughput screening for human interferon-gamma production using Homogeneous Time-Resolved Fluorescence.
  Enomoto K, Aono Y, Mitsugi T, Suzuki R, Preaudat M, Mathis G, Kominami G, Takemoto H.
  J Biomol Screening. 2000; 5: 263-268.
  Publication year: 2000

  Human & Mouse IFNγ assays

  Biochemical and cell-based assay development
• Quantitation of amyloid-beta peptides in biological milieu using a novel homogeneous time-resolved fluorescence (HTRF) assay
  Clarke EE, Shearman MS.
  J Neurosci Methods. 2000;102(1):61-8.
  Publication year: 2000

  Streptavidin & affinity reagents toolbox

  Biochemical and cell-based assay development

  
• Homogeneous proximity tyrosine kinase assays : scintillation proximity assay versus homogeneous time-resolved fluorescence.
  Park YW, Cummings RT, Wu L, Zheng S, Cameron PM, Woods A, Zaller DM, Marcy AI, Hermes JD.
  Anal Biochem. 1999;269(1):94-104.
  Publication year: 2000

  Kinase toolbox reagents
• Development of a Homogeneous Time-Resolved Fluorescence assay for high throughput screening to identify Lck inhibitors.Comparison with scintillation proximity assay and streptavidin-coated plate assay
  Ohmi N, Wingfield JM, Yazawa H, Inagaki O.
  J Biomol Screen. 2000;5(6):463-70.
  Publication year: 2000

  Kinase toolbox reagents
• Development of a binding assay for p53/HDM2 by using homogeneous time-resolved fluorescence
  Kane SA, Fleener CA, Zhang YS, Davis LJ, Musselman AL, Huang PS.
  Anal Biochem. 2000;278(1):29-38
  Publication year: 2000

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox
• Europium cryptate labeled deoxyuridine-triphosphate analog : synthesis and enzymatic incorporation
  Alpha-Bazin B, Bazin H, Guillemer S, Sauvaigo S, Mathis G.
  Nucleosides Nucleotides Nucleic Acids. 2000;19(9):1463-74
  Publication year: 2000

  HTRF® Labeling reagents toolbox
• Europium cryptate-tethered ribonucleotide for the labeling of RNA and Its detection by time-Resolved Amplification of cryptate Emission
  Alpha-Bazin B, Bazin H, Boissy L, Mathis G.
  Anal Biochem. 2000;286:17-25
  Publication year: 2000

  HTRF® Labeling reagents toolbox

  Streptavidin & affinity reagents toolbox
• Real-time monitoring of the hybridization reaction : application to the quantification of oligonucleotides in biological samples.
  Boutet V, Delaunay V, De Oliveira MC, Boquet D, Grognet JM, Grassi J, Deverre JR.
  Biochem Biophys Res Comm. 2000;268:92-8
  Publication year: 2000

  HTRF® Labeling reagents toolbox

  Streptavidin & affinity reagents toolbox
• New homogeneous reverse transcriptase and nuclease assays based on rare earth cryptate and fluorescent energy transfer.
  Alpha-Bazin B, Mathis G
  Nucleosides & Nucleotides. 1999; 18(6&7): 1277-1278
  Publication year: 1999

  Streptavidin & affinity reagents toolbox
• Mono(di)nuclear Europium(lll) complexes of macrobi(tri)cyclic cryptands derived from diazatetralactams as luminophores in aqueous solution.
  Galaup C, Picard C, Cathala B, Cazaux L, Tisns P, Autiro H, Aspe D.
  Helv Chim Acta. 1999;82:543-60
  Publication year: 1999

  HTRF® chemistry
• Application of homogeneous time-resolved fluorescence (HTRF®) to monitor poly-ubiquitination of wild-type p53
  Yabuki N, Watanabe S, Kudoh T, Nihira S, Miyamato C.
  Comb Chem High Throughput Screen. 1999 Oct;2(5):279-87.
  Publication year: 1999

  Ubiquitin assay toolbox
• Use of a phosphotyrosine-antibody pair as a general detection method in homogeneous time-resolved fluorescence: application to human immunodeficiency viral protease.
  Cummings RT, McGovern HM, Zheng S, Park YW, Hermes JD.
  Anal Biochem. 1999;269(1):79-93.
  Publication year: 1999

  Kinase toolbox reagents

  HTRF® Labeling reagents toolbox
• Stoichiometry of a ligand-gated ion channel determined by fluorescence energy transfer.
  Farrar SJ, Whiting PJ, Bonnert TP, McKernan RM.
  J Biol Chem. 1999;274(15):10100-4.
  Publication year: 1999

  HTRF® Anti-tag reagents toolbox
• HTRF technology
  Mathis G.
  J. Biomolecular Screening.1999;4:309-313
  Publication year: 1999

  HTRF® chemistry
• Development of a CD28/CD86 (B7-2) binding assay for high throughput screening by homogenous time-resolved fluorescence.
  Mellor GW, Burden MN, Preaudat M, Joseph Y, Cooksley SB, Ellis JH, Banks MN.
  J Biomol Screening. 1998;3:91-99.
  Publication year: 1998

  HTRF® Anti-species reagents toolbox
• Nuclear receptors have distinct affinities for coactivators : characterization by fluorescence resonance energy transfer
  Zhou G, Cummings R, Li Y, Mitra S, Wilkinson HA, Elbrecht A, Hermes JD, Schaeffer JM, Smith RG, Moller DE
  Mol Endocrinol. 1998;12(10):1594-604.
  Publication year: 1998

  HTRF® Anti-tag reagents toolbox

  Streptavidin & affinity reagents toolbox
• Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer.
  Mathis G
  Clin Chem. 1995; 41(9): 1391-7
  Publication year: 1995

  HTRF® chemistry
• Europium (III) Cryptate: A Fluorescent Label for the Detection of DNA Hybrids on Solid Support.
  Prat O, Lopez E, Mathis G.
  Anal Biochem. 1991;195:283-9
  Publication year: 1991

  HTRF® chemistry