HTRF® Toolbox Reagents

Cisbio Bioassays offers a comprehensive line of HTRF toolbox reagents that contains Europium-cryptate and XL-665 labeled components. These reagents bring an additional level of flexibility to assay design and are used to create new HTRF assays without the need for production of custom reagents. One of the most common means of adding flexibility, particularly when protein-specific reagents are not available, is the use of tagged biomolecules. Frequently used tags include proteinic or peptidic structures such as GST, 6HIS, c-myc, FLAG®, and HA and small organic motifs like biotin and dinitrophenyl (DNP).

Our reagent toolbox includes Europium-cryptate and XL-665 labeled anti-tag antibodies, anti-immunoglobulin antibodies, streptavidins, lectins and other affinity system reagents that provide maximum flexibility and superior performance. A diversity of assays have been developed with these reagents including protein:protein interactions, nuclear receptor assays, receptor dimerization, ligand binding, receptor internalization and enzyme assays. As with all HTRF assays, assays developed using our toolbox reagents can easily be miniaturized and are amenable to automation.