HTRF innovations

Experience Cisbio's latest developments

Unable to find an assay that suits your need or you want to convert your ELISA into a homogeneous method. Through Assays on demand, Cisbio Bioassays gives you a list of its latest developments for the Drug Discovery field. To see the extent of our expertise and know-how, and to find a solution that will fulfil your specific needs, see our service offerings.

These assays on request are sold frozen and available in 1,000 and 20,000 test volumes.

SIRT1 NEW FORMAT! Format: acetylated d2-labeled substrate recognized by a Eu(K)-labeled antibody. Signal is directly linked to enzyme activity. Maximum signal is obtained without enzyme and decreases as the enzyme removes the acetyl group from the substrate. Applications: High Throughput SIRT1 inhibitor or activator Screening. Features: Incubation time (enzymatic step): 30 min Enzyme titration EC50: 22 mU/well Z' at EC50 > 0.7 Download SIRT1 SBS 2009 poster	Aldosterone Format: competitive immunoassay Application: quantitative measurement of aldosterone and aldosterone synthase activity assessment Features: Incubation time: 1 hour at RT Detection limit: 24 pg/mL (initial) Working range: 24 pg/mL to 17 700 pg/mL EC50: 453 pg/mL Cross reactivity: Corticosterone: 0.05% 11-deoxycorticosterone: 0.0017%	Triiodothyronine Format: competitive immunoassay Application: quantitative measurement of T3 Features: Incubation time: 1 hour at RT Detection limit: 24 pg/mL (initial) Working range: 24 pg/mL to 6 000 pg/mL EC50: 464 pg/mL Cross reactivity: T2: 0.45% T4: 1%
IL-2 Format: sandwich immunoassay Application: quantitative determination of IL-2 Features: Incubation time: 2 hours at RT Detection limit: 39 pg/mL (initial concentration) Linear range: 39 to 5000 pg/mL See our complete cytokine offer	LTB4 Format: competitive immunoassay Application: quantitative measurement of LTB4 in buffer solution or cell supernatants Features: Incubation time: 2 hours at RT Detection limit: 49 pg/mL Linear range: 228 to 6180 pg/mL EC50: 1069 pg/mL Cross reactivity: 6-trans 12-epi LTB4: 0.19% 6-trans LTB4: 5.5%	Glucagon Format: competitive immunoassay Application: quantitative measurement of Glucagon Features: Incubation time: 3 hours at RT Detection limit: 50 pM (final) Working range: 62 to 555 pM final EC50: 0.23 nM final See our insulin assay
Testosterone Format: competitive immunoassay Application: measurement and quantification of Testosterone Features: Incubation time: 1H at RT S/B: 13 Detection limit: 0.1 nM initial Working range: 0.1 to 10 nM EC50: 0.84 nM	Mouse IL17 Format: sandwich immunoassay Application: quantitative determination of mouse IL17 Features: Incubation time: 2H at RT Detection limit: 10 pg/mL (diluent) and 20 pg/mL (culture medium) Linear range: 15.5 pg/mL to 2000 pg/mL

SIRT1 NEW FORMAT!

Format: acetylated d2-labeled substrate recognized by a Eu(K)-labeled antibody. Signal is directly linked to enzyme activity. Maximum signal is obtained without enzyme and decreases as the enzyme removes the acetyl group from the substrate.

Applications: High Throughput SIRT1 inhibitor or activator Screening.

Features:

Incubation time (enzymatic step): 30 min
Enzyme titration EC50: 22 mU/well
Z' at EC50 > 0.7

Download SIRT1 SBS 2009 poster

Aldosterone

Format: competitive immunoassay

Application: quantitative measurement of aldosterone and aldosterone synthase activity assessment

Features:

Incubation time: 1 hour at RT
Detection limit: 24 pg/mL (initial)
Working range: 24 pg/mL to 17 700 pg/mL
EC50: 453 pg/mL
Cross reactivity:

Corticosterone: 0.05%
11-deoxycorticosterone: 0.0017%

Triiodothyronine

Format: competitive immunoassay

Application: quantitative measurement of T3

Features:

Incubation time: 1 hour at RT
Detection limit: 24 pg/mL (initial)
Working range: 24 pg/mL to 6 000 pg/mL
EC50: 464 pg/mL
Cross reactivity:

T2: 0.45%
T4: 1%

IL-2

Format: sandwich immunoassay

Application: quantitative determination of IL-2

Features:

Incubation time: 2 hours at RT
Detection limit: 39 pg/mL (initial concentration)
Linear range: 39 to 5000 pg/mL See our complete cytokine offer

LTB4

Format: competitive immunoassay

Application: quantitative measurement of LTB4 in buffer solution or cell supernatants

Features:

Incubation time: 2 hours at RT
Detection limit: 49 pg/mL
Linear range: 228 to 6180 pg/mL
EC50: 1069 pg/mL
Cross reactivity:

6-trans 12-epi LTB4: 0.19%
6-trans LTB4: 5.5%

Glucagon

Format: competitive immunoassay

Application: quantitative measurement of Glucagon

Features:

Incubation time: 3 hours at RT
Detection limit: 50 pM (final)
Working range: 62 to 555 pM final
EC50: 0.23 nM final See our insulin assay

Testosterone

Format: competitive immunoassay

Application: measurement and quantification of Testosterone

Features:

Incubation time: 1H at RT
S/B: 13
Detection limit: 0.1 nM initial
Working range: 0.1 to 10 nM
EC50: 0.84 nM

Mouse IL17

Format: sandwich immunoassay

Application: quantitative determination of mouse IL17

Features:

Incubation time: 2H at RT
Detection limit: 10 pg/mL (diluent) and 20 pg/mL (culture medium)
Linear range: 15.5 pg/mL to 2000 pg/mL

Ordering Info:

Name	Size	Cat nb
Aldosterone kit	1,000	64ALDPEB
Aldosterone bulk kit	20,000	64ALDPEC
T3 kit	1,000	64HT3PEB
T3 bulk kit	20,000	64HT3PEC
Human IL-2 kit	1,000	64IL2PEB
Human IL-2 bulk kit	20,000	64IL2PEC
LTB4 kit	1,000	64LB4PEB
LTB4 bulk kit	20,000	64LB4PEC
Glucagon kit	1,000	64GLUPEB
Glucagon bulk kit	20,000	64GLUPEC
SIRT1 kit	1,000	64SI1PEB
SIRT1 bulk kit	20,000	64SI1PEC
Testosterone kit	1,000	64TESPEB
Testosterone bulk kit	20,000	64TESPEC
Mouse IL17 kit	1,000	64M17PEB
Mouse IL17 bulk kit	20,000	64M17PEC

For more information or To Place an Order:

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