Assays on demand

Experience Cisbio Bioassays's latest innovations

You cannot find an assay that fits your needs perfectly or you want to convert your ELISA into an homogeneous method? Through Assays on demand, Cisbio Bioassays gives you a list of its latest developments for the Drug Discovery field. To see the extent of our expertise and know-how, and find a solution to your specific needs, see our service offer.

These assays on request are sold frozen and available in 1,000 and 20,000 test volumes.

SIRT1 NEW! Format: Acetyl-biotinylated substrate recognized by a Eu3+ anti-acetyl MAb and SA-XL665. The level of enzymatic activity is inversely proportional to the amount of signal produced in the system. Applications: High Throughput SIRT1 inhibitor or activator Screening. Features: Incubation time (enzymatic step): 30 min Enzyme titration EC50: 22 mU/well Z' at EC50 > 0.7 View SBS 2008 poster Under development: extension to other HDACs!	Aldosterone Format: competitive immunoassay Application: quantitative measurement of aldosterone and aldosterone synthase activity assessment Features: Incubation time: 1 hour at RT Detection limit: 24 pg/mL (initial) Working range: 24 pg/mL to 10 000 pg/mL EC50: 453 pg/mL Cross reactivity: Corticosterone: 0.05% 11-deoxycorticosterone: 0.0017%	Triiodothyronine Format: competitive immunoassay Application: quantitative measurement of T3 Features: Incubation time: 1 hour at RT Detection limit: 24 pg/mL (initial) Working range: 24 pg/mL to 10 000 pg/mL EC50: 464 pg/mL Cross reactivity: T2: 0.45% T4: 1%
IL-2 Format: sandwich immunoassay Application: quantitative determination of IL-2 Features: Incubation time: 2 hours at RT Detection limit: 39 pg/mL (initial concentration) Linear range: 39 to 5000 pg/mL See our complete cytokine offer	LTB4 Format: competitive immunoassay Application: quantitative measurement of LTB4 in buffer solution or cell supernatants Features: Incubation time: 2 hours at RT Detection limit: 49 pg/mL Linear range: 228 to 6180 pg/mL EC50: 1069 pg/mL Cross reactivity: 6-trans 12-epi LTB4: 0.19% 6-trans LTB4: 5.5%	Glucagon Format: competitive immunoassay Application: quantitative measurement of Glucagon Features: Incubation time: 3 hours at RT Detection limit: 50 pM (final) Linear range: 62 to 555 pM final EC50: 0.23 nM final See our insulin assay

SIRT1 NEW!

Format: Acetyl-biotinylated substrate recognized by a Eu3+ anti-acetyl MAb and SA-XL665. The level of enzymatic activity is inversely proportional to the amount of signal produced in the system.

Applications: High Throughput SIRT1 inhibitor or activator Screening.

Features:

Incubation time (enzymatic step): 30 min
Enzyme titration EC50: 22 mU/well
Z' at EC50 > 0.7

View SBS 2008 poster

Under development: extension to other HDACs!

Aldosterone

Format: competitive immunoassay

Application: quantitative measurement of aldosterone and aldosterone synthase activity assessment

Features:

Incubation time: 1 hour at RT
Detection limit: 24 pg/mL (initial)
Working range: 24 pg/mL to 10 000 pg/mL
EC50: 453 pg/mL
Cross reactivity:

Corticosterone: 0.05%
11-deoxycorticosterone: 0.0017%

Triiodothyronine

Format: competitive immunoassay

Application: quantitative measurement of T3

Features:

Incubation time: 1 hour at RT
Detection limit: 24 pg/mL (initial)
Working range: 24 pg/mL to 10 000 pg/mL
EC50: 464 pg/mL
Cross reactivity:

T2: 0.45%
T4: 1%

IL-2

Format: sandwich immunoassay

Application: quantitative determination of IL-2

Features:

Incubation time: 2 hours at RT
Detection limit: 39 pg/mL (initial concentration)
Linear range: 39 to 5000 pg/mL See our complete cytokine offer

LTB4

Format: competitive immunoassay

Application: quantitative measurement of LTB4 in buffer solution or cell supernatants

Features:

Incubation time: 2 hours at RT
Detection limit: 49 pg/mL
Linear range: 228 to 6180 pg/mL
EC50: 1069 pg/mL
Cross reactivity:

6-trans 12-epi LTB4: 0.19%
6-trans LTB4: 5.5%

Glucagon

Format: competitive immunoassay

Application: quantitative measurement of Glucagon

Features:

Incubation time: 3 hours at RT
Detection limit: 50 pM (final)
Linear range: 62 to 555 pM final
EC50: 0.23 nM final See our insulin assay

Ordering Info:

Name	Size	Cat nb
Aldosterone kit	1,000	64ALDPEB
Aldosterone bulk kit	20,000	64ALDPEC
T3 kit	1,000	64HT3PEB
T3 bulk kit	20,000	64HT3PEC
IL-2 kit	1,000	64IL2PEB
IL-2 bulk kit	20,000	64IL2PEC
LTB4 kit	1,000	64LB4PEB
LTB4 bulk kit	20,000	64LB4PEC
Glucagon kit	1,000	64GLUPEB
Glucagon bulk kit	20,000	64GLUPEC
SIRT1	1,000	64SR1PEB
SIRT1	20,000	64SR1PEC

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