HTRF®Labeling Reagents
For do-it-yourself labeling
HTRF® labeling reagents are a do-it-yourself solution for labeling biomolecules with donor and acceptor fluorophores as well as chemical tags. These reagents are ideal for experienced users familiar with labeling and chromatography. The availability of various functional groups on Eu3+ Cryptate and XL665 adds significant flexibility to labeling strategies and assay design. If a do-it-yourself solution isn't for you, Cisbio Bioassays's labeling experts are available to you through our custom labeling service.
- Large variety of labeling reagents available for complete assay design (donor, acceptor, DNP)
- Long reagent shelf life
- Choice of functional groups for simplified labeling
- Labeling of biomolecules with Cryptate, XL665 or DNP
- Bulk labelings
Cryptate Reagents:
Europium Trisbipyridine Cryptate (Eu3+-TBP Cryptate) is available from Cisbio Bioassays in microgram to milligram quantities for use by customers in biomolecule labeling. The cryptate is available with 3 different functional groups:
K-NH2:
Di-amino Cryptate (Eu3+-TBP-NH2 Cryptate), for use in combination with bi-functional reagents such as
carbodiimides and SMCC/SPDP, for a flexible labeling solution
K-NHS:
N-hydroxysuccinimide activated Cryptate (Eu3+-TBP-NHS Cryptate), reacts with primary amines. This
specific Cryptate is available either by itself or in the Cryptate labeling kit.
K-MP:
Maleimide activated Cryptate (Eu3+-TBP-MP Cryptate), enabling specific labeling of thiol groups.
XL665 Reagents:
Cross-linked allophycocyanin, XL665, is available either with an amine group (XL665-NH2) or a maleimide group (XL665-maleimide) for simplified conjugation.
DNP Chemical Tag:
DNP (2,4-dinitrophenyl) is a small organic tagging alternative to biotin. It can be detected using an anti-DNP antibody (see HTRF toolbox reagents). The DNP-NHS labeling reagent enables conjugation of DNP by targeting primary amino-groups (N-terminus or lysine residues) under very mild conditions.
SNAP-vitro HTRF Kits:
SNAP-tag is a revolutionary protein tag from Covalys which allows the covalent attachment of virtually any molecule to the tag. A protein of interest is expressed as a SNAP-tag fusion. The SNAP-tag then reacts with a substrate of interest, such as a fluorophore, to form a highly stable conjugate. SNAP-vitro HTRF® kits are available from Covalys and enable the labeling of SNAP-tag fusion proteins with Europium-Cryptate or XL665 for use in HTRF® assays.
Ordering Info:
Individual Labeling Reagents:
| Description | Label quantity mg (nmoles) |
Target molecule* (mg) |
Catalog no.* | Product Insert |
| Eu3+-TBP-NH2 Cryptate | 0.1 (68) 1 (680) |
0.8 8 |
65EU2ABA 65EU2ABB |
— |
| Eu3+-TBP-NHS Cryptate | 0.1 (69) 1 (690) |
0.8 8 |
65EUSABA 65EUSABB |
— |
| Eu3+-TBP-MP Cryptate | 0.1 (66) 1 (660) |
0.8 8 |
65EUMABA 65EUMABB |
— |
| XL665-NH2 | 1 (10) 10 (100) |
0.6 6 |
65XL2ABB 65XL2ABC |
— |
| XL665-Maleimide | 1 (10) 10 (100) |
0.6 6 |
65XLMABB 65XLMABC |
— |
| DNP-NHS | 0.2 (500) 1 (2,500) |
7.5 37.5 |
65DNSABD 65DNSABB |
|
| * Quantity of target molecule that can be labeled is based on a MW 150kDa (antibody) biomolecule | ||||
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
