HTRF® Transcreener® ADP

For Kinase and ATPase screening

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The HTRF® Transcreener® ADP assay is based on our patented HTRF® technology for the direct detection of ADP using a monoclonal antibody labeled with Eu3+ cryptate. HTRF is an enzyme-free detection chemistry thus producing fewer false positives. This assay can be used for high throughput screening or profiling of any ATP/ADP dependent target such as serine/threonine and tyrosine kinases as well as lipid kinases and chaperonins.

Features:
  • Monoclonal antibody specific to ADP
  • Homogeneous non-radioactive screening alternative
  • Enzyme-free detection technology
  • Compatible with any substrate
Applications:
  • Kinases and ATPases
  • High throughput Screening
  • Profiling
  • Mechanistic studies





Assay Principle:

 

The HTRF® Transcreener® ADP assay is a competitive immunoassay in which native ADP and d2-labeled ADP compete for binding to a monoclonal anti-ADP labeled with Eu3+ cryptate. The assay consists of two steps: an enzymatic step followed by a universal detection step.

Enzymatic step:

  • For kinases, the substrate is incubated with the kinase. Then, the addition of ATP allows the enzymatic reaction to start: the kinase phosphorylates the substrate.
  • For ATPases, ATP will be converted into ADP and inorganic phosphate.

Detection step:
The anti-ADP labeled with Eu3+ cryptate will compete with native ADP and d2-labeled ADP. As a result, the signal is inversely proportional to the concentration of ADP in the sample.

               


Ordering Info:

 

 

Description Quantity* Cat no. Product Insert
HTRF® Transcreener® ADP 1,000 tests
20,000 tests		 64ADPPEB
64ADPPEC		 pdf
pdf

Companion Products:

Description Quantity* Cat no.
HTRF® Transcreener® ADP enzymatic buffer 5x 10mL 62ZB1FDC
HTRF® Transcreener® ADP enzymatic buffer 5x 50mL 62ZB1FDD
HTRF® Transcreener® ADP detection buffer 13mL 62DB1RDD
HTRF® Transcreener® ADP detection buffer 200mL 62DB1RDF

Transcreener® HTS Assay Platform technology is patented (U.S. patent 7,332,278), and is being used under license from Bellbrook Labs, LLC.



For more information or To Place an Order:
 

U.S. and Canada
1-888-963-4567

Find your local sales and support team

Europe and other countries
+33(0)466 796 705

Japan
+81-3-5510-2932