cAMP Cell-Based Assays
Cisbio Bioassays offers a line of three ready-to-use assays for measuring cAMP using HTRF® technology. Each kit is optimized for detecting a specific range of cAMP concentrations to allow fine tuning of assay sensitivity. These kits enable high quality, cell-based assay in 96-well or high throughput screening formats (384- or 1536-well).
- Low false positive, false negative results
- Stable EC50 from 30 minutes to 7 days
- Miniaturization down to <10µl
- Compatible with fresh or frozen cells
- Gs or Gi coupled receptor activation
- Agonist / antagonist screening
- Phosphodiesterase (PDE) monitoring
- Adenylate cyclase activity assessment
Assay Principle:
cAMP kits are based on a competitive immunoassay using cryptate-labeled anti-cAMP and d2-labeled cAMP. d2 is our newest HTRF acceptor fluorophore whose small size eliminates EC50 drift.
To also satisfy all the robotics constraints, each kits are supplied with a 1 or 2 dispensing steps protocol after cell stimulation
Product Specifications:
Three kit groups to cover a broad range of cell-based assays.
With the introduction of Lumi4®-Tb cryptate, cAMP femto, the most sensitive cAMP assay is now available with two cryptates (europium or terbium). Both kits enable the detection of as little as 28 pM cAMP (0.6 fmoles/well in 20µl volume). This allows use of very low cell densities, 100 to 500 cells per well.
The cAMP dynamic 2 - wide range of cAMP concentrations ideal for a broad range of Gi/s assays.
The cAMP HiRange - well suited for HTS with extended signal-to-background ratios (Z' > 0.9) and for use with membrane preparations.
| Cryptate | S/B | IC20 nM* | IC50 nM* | IC80 nM* | |
| cAMP femto 2 Kit | Eu3+ | 10 | 0.33 | 1.76 | 9.4 |
| cAMP femto Tb Kit | Tb2+ | 10 | 0.33 | 1.76 | 9.4 |
| cAMP dynamic 2 Kit | Eu3+ | 18 | 0.91 | 4.07 | 18.1 |
| cAMP HiRange Kit | Eu3+ | 41 | 4.27 | 22.8 | 121 |
| * final cAMP concentration | |||||
Ordering Info:
cAMP kit - 384-well assay format (20 µl)
| Description | Quantity | Cat no. | Product Insert | MSDS |
| cAMP femtomolar Tb | 1,000 tests | 62AM7PEB | ||
| 20,000 tests | 62AM7PEC | |||
| 100,000 tests | 62AM7PEJ | Request MSDS | ||
| cAMP femtomolar | 1,000 tests | 62AM5PEB | ||
| 20,000 tests | 62AM5PEC | |||
| 100,000 tests | 62AM5PEJ | |||
| cAMP dynamic | 1,000 tests | 62AM4PEB | ||
| 20,000 tests | 62AM4PEC | |||
| 100,000 tests | 62AM4PEJ | |||
| 1,000,000 tests | 62AM4PEM | |||
| cAMP HiRange | 1,000 tests | 62AM6PEB | ||
| 20,000 tests | 62AM6PEC | |||
| 100,000 tests | 62AM6PEJ | |||
| 1,000,000 tests | 62AM6PEM |
cAMP kit - 96-well assay format (100 µl)
| Description | Quantity | Cat no. | Product Insert | MSDS |
| cAMP dynamic 2 kit | 2x96 tests | 62AM4PEF | - |
Companion Products:
| Description | Cat no. | MSDS |
| cAMP femto 2 calibrator | 62AM5CDA | |
| cAMP femto 2 control | 62AM5TDA | |
| cAMP dynamic 2 calibrator | 62AM4CDA | |
| cAMP dynamic 2 control | 62AM4TDA | |
| cAMP HiRange calibrator | 62AM6CDA | |
| cAMP HiRange control | 62AM6TDA | |
| Diluent for standard curve prep. | 62DL1DDD |
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
