Histamine H1 receptor ligand binding assay
Histamine H1 binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Histamine H1 receptor green inv ago-5,000 tests | L0015GRE | ||
| Tag-lite Histamine H1 - 1 Million labeled cells | C1TT1H1 | - | |
| Tag-lite buffer - 100 ml (5X) | LABMED | - | - |
The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 100 tests at a cell density of 10,000 cells/well.
- This inverse agonist is a Mepyramine derivative labeled with a Green Emitting HTRF fluorescent probe
- This ligand has been successfully used in a Histamine H1 receptor Tag-lite binding assay
- Developed under collaboration with CellAura
- Concentration: 20µM
- Volume: 250µL
- Buffer: HEPES 50mM, pH7.4 + 10%DMSO
- Molecular Weight: 874
| Related products | Product reference | Product insert |
| Plasmid pSNAP-H1 | PSNAPH1 | |
| Tag-lite SNAP-Lumi4Tb - 2 nmoles | SSNPTBC | - |
Assay Validation:
Histamine H1 receptor ligand binding assay was performed using the starter pack reagents.
Kd was determined, and signal inhibition (IC50) by agonist and antagonist was performed with L0015GRE (Tag-lite Histamine H1 receptor green inverse agonist) 50nM.
Results obtained with radioligand assay and Tag-lite platform were very similar.
| Family | Receptor | Compounds (antagonist) | Radioactivity (Ki nM) |
Tag-lite (Ki nM) |
| Histamine | H1 | Triprolidine | 1.53 (1) | 1.51 |
| Doxepin | 0.32 (1) | 0.65 |
Booth et al, J Pharmacol Exp Ther, 2002
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
