GIPR receptor ligand binding assay
Glucagon GIPR binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Tag-lite GIP receptor red agonist - 5,000 tests | L0018RED | ||
| Tag-lite Glucagon GIPR - 1 Million labeled cells | C1TT1GIPR | - | |
| Tag-lite buffer - 100 ml (5X) | LABMED | - | - |
The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 100 tests at a cell density of 10,000 cells/well.
- This agonist is a GIP derivative labeled with a Red Emitting HTRF fluorescent probe
- This ligand has been successfully used in a GIP receptor Tag-lite binding assay
- Concentration: 10µM
- Volume: 100µL
- Buffer: H2O
- Molecular Weight: 5851.4
| Related products | Product reference | Product insert |
| Plasmid pSNAP-GIPR | PSNAPGIPR | |
| Tag-lite SNAP-Lumi4Tb - 2 nmoles | SSNPTBC | - |
Assay Validation:
GIPR receptor ligand binding assay was performed using the starter pack reagents.
Kd was determined, and signal inhibition (IC50) by agonist and antagonist was performed with L0018RED (Tag-lite Glucagon receptors red agonist) 10nM.
Results obtained with radioligand assay and Tag-lite platform were very similar
| Family | Receptor | Coumpound (agonist) | Radioactivity (Ki nM) |
Tag-lite (Ki nM) |
| Glucagon | GIPR | GIP | 2.37 (1) | 3.50 |
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
