GHSR1 A receptor ligand binding assay
GHSR1 binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Tag-lite Ghrelin 1A receptor red agonist - 5,000 tests | L0020RED | ||
| Tag-lite GHSR - 1 Million labeled cells | C1TT1GHSR1A | ||
| Tag-lite buffer - 100 ml (5X) | LABMED | - |
The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 200 tests at a cell density of 5,000 cells/well.
Features:
- This antagonist is a ghrelin derivative labeled with a red emitting HTRF fluorescent probe
- This ligand has been successfully used in a Ghrelin 1A receptor Tag-lite binding assay.
- Concentration: 10µM
- Volume: 30µL
- Buffer: H2O
- Molecular Weight: 4122.2
Assay Validation:
| Family | Receptor | Coumpounds | Radioactivity | Tag-lite (Ki nM) |
| Ghrelin | GHSR1A | Ghrelin (agonist) | IC50 = 10.5 (1) | 20.6 |
| MK0677 (antagonist) | Ki = 0.7 nM (2) | 0.69 |
(1)Craenenbroeck et al. Peptides, 2004
(2)Mc Kee et al. Mol Endocrinol, 1997
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
