GABA-B receptor ligand binding assay
GABA-B binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Tag-lite GABAB receptor red antagonist - 5,000 tests | L0014RED | ||
| Tag-lite GABA-B - 1 Million labeled cells | C1TT1GABAB | - | |
| Tag-lite buffer - 100 ml (5X) | LABMED | - | - |
The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 100 tests at a cell density of 10,000 cells/well.
- This antagonist is a CGP-54626 derivative labeled with a red emitting HTRF fluorescent probe
- This ligand has been successfully used in a GABAB receptor Tag-lite binding assay
- Developed under collaboration with CellAura
- Concentration: 10µM
- Volume: 250µL
- Buffer: HEPES 50mM, pH7.4 + 10% DMSO
- Molecular Weight: 1044
| Related products | Product reference | Product insert |
| Plasmid pSNAP-GABA-B | PSNAPGABAB | |
| Tag-lite SNAP-Lumi4Tb - 2 nmoles | SSNPTBC | - |
Assay Validation:
GABA-B receptor ligand binding assay was performed using the starter pack reagents.
Kd was determined, and signal inhibition (IC50) by agonist and antagonist was performed with L0014RED (Tag-lite GABAB receptors red antagonist) 25 nM.
Results obtained with radioligand assay and Tag-lite platform were very similar.
| Family | Receptor | Compounds | Radioactivity (Ki nM) |
Tag-lite (Ki nM) |
| GABA | GABA-B | CGP-5426 | 8.13 (1) | 3.9 |
(1) Woods et al Br J Pharmacol, 2000
For more information or To Place an Order:
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+33(0)466 796 705
