Delta opioid receptor ligand binding assay
Opioid receptors are currently classified into three groups, the κ, δ and µ receptors. Subtypes within each group have been suggested, including µ1, µ2, δ1, δ2, κ1, κ2, and κ3.
An opioid-like receptor with a high degree of homology to µ, δ and κ-opioid receptors has been cloned and termed the orphan opioid receptor (NOP, ORL1). This receptor has a pharmacological profile distinct from the classical opioid receptors and an endogenous ligand, nociceptin (orphanin FQ) has been identified.
Delta opioid binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Tag-lite Opioid receptors red antagonist - 5,000 tests | L0005RED | ||
| Tag-lite Delta opioid - 1 Million labeled cells | C1TT1DOP | ||
| Tag-lite buffer - 100 ml (5X) | LABMED | - |
The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 200 tests at a cell density of 5,000 cells/well.
- This antagonist is naltrexone derivative labeled with a red Emitting HTRF fluorescent probe.
- This ligand has been successfully used both in a µ, δ and κ-opiod Tag-lite binding assays.
- Buffer: HEPES pH7.4 + 10% DMSO
- Molecular Weight: 1144.4
| Related products | Product reference | Product insert |
| Plasmid pSNAP-Delta opioid | PSNAPDOP | |
| Tag-lite SNAP-Lumi4Tb - 2 nmoles | SSNPTBC |
* The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 200 tests at a cell density of 5,000 cells/well.
Assay Validation:
Delta opioid receptor ligand binding assay was performed using the starter pack reagents.
Kd was determined and signal inhibition (IC50) by antagonists was performed with L0005RED (Tag-lite opioid receptors red antagonist) 8 nM.
Results obtained with radioligand assay and Tag-lite platform were very similar.
| Family | Receptor | Compounds (antagonist) | Radioactivity( Ki nM) | Tag-lite (Ki nM) |
| Opioid | delta | Naloxone | 36.9 (1) | 37.4 |
| Naltrindole | 0.033 (2) | 0.089 |
(1) Heather. et al. Anal Biochem, 2005
(2) Korlipara et al. Eur J. Med Chem, 1997
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
