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CXCR4 receptor ligand binding assay

Chemokine receptor CXCR4 plays a key role in lymphocyte homing and hematopoiesis as well as in breast cancer metastasis.

CXCR4 receptor binding kit

The ligand binding kit contains all the material to perform at least 120 tests (labeled cells, fluorescent ligands and Tag-lite buffer).
Ligand binding kit Product reference Product insert
Tag-lite CXCR4 binding kit - 120 tests 82CXCR4LBPEA pdf

CXCR4 ligand binding products

The following ligand binding products may be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 100 tests at a cell density of 10,000 cells/well.
Ligand binding products Product reference Product insert MSDS
Tag-lite CXCR4 receptor red agonist - 5,000 tests L0012RED pdf pdf
Tag-lite CXCR4 - 1 Million labeled cells C1TT1CXCR4 pdf -
Tag-lite buffer - 100 ml (5X) LABMED - -
Related products Product reference Product insert
Tag-lite CXCR4 stable cell line C1SU1CXCR4 pdf
Plasmid pSNAP-CXCR4 PSNAPCXCR4 pdf
Tag-lite SNAP-Lumi4Tb - 2 nmoles SSNPTBC -
Features:
  • This agonist is SDF1α derivative labeled with a red emitting HTRF fluorescent probe
  • This ligand has been successfully used in a CXCR4 Tag-lite binding assay
  • Concentration: 10µM
  • Buffer: H2O
  • Molecular Weight: 8445.6

Assay validation on Tag-lite CXCR4 stable cell line:

 

Chemokine CXCR4 receptor binding assay was performed using the binding kit reagents

Assay format shows particularly large signal assay windows, Kd and IC50 obtained are consistent with data already published.

Tag-lite CXCR4 binding assays validation on cell line - Kd determination Tag-lite CXCR4 binding assays validation on cell line - competition assay
Family Receptor Compounds Radioactivity( Ki nM) Tag-lite (Ki nM)
Chemokine CXCR4 SDF1-α (agonist) 24 (1) 18
AMD3100 (antagonist) 106 (2) 25

(1) Fricker et al. Biochem Pharm 2006
(2) Zhang et al. JBC 2002



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