CXCR4 receptor ligand binding assay
Chemokine receptor CXCR4 plays a key role in lymphocyte homing and hematopoiesis as well as in breast cancer metastasis.
CXCR4 receptor binding kitThe ligand binding kit contains all the material to perform at least 120 tests (labeled cells, fluorescent ligands and Tag-lite buffer). |
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| Ligand binding kit | Product reference | Product insert | |
| Tag-lite CXCR4 binding kit - 120 tests | 82CXCR4LBPEA | ||
CXCR4 ligand binding productsThe following ligand binding products may be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 100 tests at a cell density of 10,000 cells/well. |
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| Ligand binding products | Product reference | Product insert | MSDS |
| Tag-lite CXCR4 receptor red agonist - 5,000 tests | L0012RED | ||
| Tag-lite CXCR4 - 1 Million labeled cells | C1TT1CXCR4 | - | |
| Tag-lite buffer - 100 ml (5X) | LABMED | - | - |
| Related products | Product reference | Product insert | |
| Tag-lite CXCR4 stable cell line | C1SU1CXCR4 | ||
| Plasmid pSNAP-CXCR4 | PSNAPCXCR4 | ||
| Tag-lite SNAP-Lumi4Tb - 2 nmoles | SSNPTBC | - | |
Features:
- This agonist is SDF1α derivative labeled with a red emitting HTRF fluorescent probe
- This ligand has been successfully used in a CXCR4 Tag-lite binding assay
- Concentration: 10µM
- Buffer: H2O
- Molecular Weight: 8445.6
Assay validation on Tag-lite CXCR4 stable cell line:
Chemokine CXCR4 receptor binding assay was performed using the binding kit reagents
Assay format shows particularly large signal assay windows, Kd and IC50 obtained are consistent with data already published.
| Family | Receptor | Compounds | Radioactivity( Ki nM) | Tag-lite (Ki nM) |
| Chemokine | CXCR4 | SDF1-α (agonist) | 24 (1) | 18 |
| AMD3100 (antagonist) | 106 (2) | 25 |
(1) Fricker et al. Biochem Pharm 2006
(2) Zhang et al. JBC 2002
For more information or To Place an Order:
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