B2 receptor ligand binding assay
Bradykinin B2 binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Tag-lite Bradykinin B2 receptor red antagonist - 5,000 tests | L0017RED | ||
| Tag-lite Bradykinin B2 - 1 Million labeled cells | C1TT1B2 | - | |
| Tag-lite buffer - 100 ml (5X) | LABMED | - | - |
The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 100 tests at a cell density of 10,000 cells/well.
- This antagonist is a HOE140 derivative labeled with a red emitting HTRF fluorescent probe
- This ligand has been successfully used in a Bradykinin B2 receptor Tag-lite binding assay
- Concentration: 10µM
- Volume: 100µL
- Buffer: H2O + 10% MeCN
- Molecular Weight: 1929.8
| Related products | Product reference | Product insert |
| Plasmid pSNAP-B2 | PSNAPB2 | |
| Tag-lite SNAP-Lumi4Tb - 2 nmoles | SSNPTBC | - |
Assay Validation:
B2 receptor ligand binding assay was performed using the starter pack reagents.
Kd was determined, and signal inhibition (IC50) by agonist and antagonist was performed with L00017RED (Tag-lite Bradykinin B2 receptors red antagonist) 10nM.
Results obtained with radioligand assay and Tag-lite platform were very similar.
| Family | Receptor | Compounds | Radioactivity (Ki nM) |
Tag-lite (Ki nM) |
| Bradykinin | B2 | HOE (antagonist) | 0.22 (1) | 0.46 |
| WIN 64338 (antagonist) | 211.2 (1) | 271.1 | ||
| Lys-Bradykinin (agonist) | 28 (1) | 59.6 |
(1)Robert et al. Br J Pharmacol, 2002
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
