Bradykinin B1 receptor ligand binding assay
Bradykinin B1 binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Tag-lite Bradykinin B1 receptor red antagonist | L0016RED | - | |
| Tag-lite Bradykinin B1 - 1 Million labeled cells | C1TT1B1 | ||
| Tag-lite buffer - 100 ml (5X) | LABMED | - |
Features:
- Tag-lite Bradykinin B1 receptor red antagonist is a B9958 derivative labeled with a red emitting HTRF fluorescent probe
- This ligand has been successfully used in a Bradykinin B1 receptor Tag-lite binding assay
- Volume/Concentation; 30 µL, 10µM (H20+10%DMSO)
- Molecular Weight: 2062.8
Assay Validation:
Bradykinin B1 receptor ligand binding assay is performed by using the starter pack reagents.
Kd was determined, and signal inhibition (IC50) by agonist and antagonist was performed with L0016RED (Tag-lite Bradykinin receptor red antagonist) 1nM.
Results obtained with radioligand assay and Tag-lite platform were very similar.
| Family | Receptor | Coumpounds | Radioactivity (Ki nM) |
Tag-lite (Ki nM) |
| Bradykinin | B1 | B-9958 (antagonist, peptide) | 0.055 nM (1) | 0.12 nM |
| DesArg10-Kallidin (agonist - peptide) | 0.17 nM (2) | 0.24 nM | ||
| Kallidin (agonist - peptide) | 88 nM (2) | 34.8 nM |
(1) Gera et al. J Pharmacol Exp Ther, 2006
(2) Austin et al. J Biol Chem
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