Bradykinin B1 receptor ligand binding assay
Bradykinin B1 binding assay starter pack
| Products | Product reference | Product insert |
| Tag-lite Bradykinin B1 receptor red antagonist | L0016RED | |
| Tag-lite Bradykinin B1 - 1 Million labeled cells | C1TT1B2 | contact us |
| Tag-lite buffer - 100 ml (5X) | LABMED | - |
The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 100 tests at a cell density of 10,000 cells/well.
- Tag-lite Bradykinin B1 receptor red antagonist is a B9958 derivative labeled with a red emitting HTRF fluorescent probe
- This ligand has been successfully used in a Bradykinin B1 receptor Tag-lite binding assay
- Volume/Concentation; 30 µL, 10µM (H20+10%DMSO)
- Molecular Weight: 2062.8
| Related products | Product reference | Product insert |
| Plasmid pSNAP-B1 | PSNAPB1 | |
| Tag-lite SNAP-Lumi4Tb - 2 nmoles | SSNPTBC | - |
Assay Validation:
Bradykinin B1 receptor ligand binding assay is performed by using the starter pack reagents.
Kd was determined, and signal inhibition (IC50) by agonist and antagonist was performed with L0016RED (Tag-lite Bradykinin receptor red antagonist) 1nM.
Results obtained with radioligand assay and Tag-lite platform were very similar.
| Family | Receptor | Compounds | Radioactivity (Ki nM) |
Tag-lite (Ki nM) |
| Bradykinin | B1 | B-9958 (antagonist, peptide) | 0.055 nM (1) | 0.12 nM |
| DesArg 10-Kallidin (agonist - peptide) | 0.17 nM (2) | 0.24 nM | ||
| Kallidin (agonist - peptide) | 88 nM (2) | 34.8 nM |
(1) Gera et al. J Pharmacol Exp Ther, 2006
(2) Austin et al. J Biol Chem
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
