Bombesin B2 receptor ligand binding assay
Bombesin B2 binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Tag-lite Bombesin receptors red agonist - 5,000 tests | L0019RED | - | |
| Tag-lite Bombesin B2 - 1 Million labeled cells | C1TT1BB2 | - | |
| Tag-lite buffer - 100 ml (5X) | LABMED | - |
Features:
- Tag-lite Bombesin receptors red agonist is a Bombesin derivative labeled with a red emitting HTRF fluorescent probe
- This ligand has been successfully used in a Bombesin BB2 receptor Tag-lite binding assay
- Quantity/Concentation; 0.15 nmol (dried). Suspend in 30µL DMSO to give a 5µ.M solution
- Molecular Weight: 2375.3
Assay Validation:
Bombesin B2 receptor ligand binding assay is performed by using the starter pack reagents.
Kd was determined, and signal inhibition (IC50) by agonist and antagonist was performed with L0019RED (Tag-lite Bombesin receptors red agonist) 1nM.
Results obtained with radioligand assay and Tag-lite platform were very similar
| Family | Receptor | Coumpounds | Radioactivity (Ki nM) |
Tag-lite (Ki nM) |
| Bombesin | BB2 | PD 168,368 (antagonist, organic) | 1173 nM (1) | 221.4 nM |
| [Pro1]-Bombesin (agonist-peptide) | 0.36 nM for Bombesin (2) | 0.24 nM |
(1) Ryan et al. JPET, 1999
(2) Mantey et al. JPET, 2006
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
