Beta2 receptor ligand binding assay
Adrenergic beta 2 binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Beta2 adrenergic receptor green antago - 5000 tests | L0011GRE | ||
| Tag-lite Adrenergic beta2 - 1 Million labeled cells | C1TT1BETA2 | - | |
| Tag-lite buffer - 100 ml (5X) | LABMED | - | - |
The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cell pack, to achieve a minimum of 100 tests at a cell density of 10,000 cells/well.
- This beta2 adrenoceptor antagonist is a propranolol derivative labeled with a green emitting HTRF fluorescent probe. This product was co-developed with CellAura Technologies to be used in conjunction with Cisbio's Tag-lite® technology platform.
- This ligand has been successfully used in a beta2-adrenergic Tag-lite binding assay
- Concentration: 10µM
- Buffer: HEPES pH7.4 + 10% DMSO
- Molecular Weight: 760
| Related products | Product reference | Product insert |
| Plasmid pSNAP-Beta2 | PSNAPBD2 | |
| Tag-lite SNAP-Lumi4Tb - 2 nmoles | SSNPTBC | - |
Assay Validation:
Beta2 receptor ligand binding assay was performed using the starter pack reagents.
Kd was determined and signal inhibition (IC50) by agonist and antagonist was performed with L0011GRE (Tag-lite beta adrenergic receptor green antagonist) 6nM. Results obtained with radioligand assay and Tag-lite platform were very similar.
| Family | Receptor | Compounds | Radioactivity( Ki nM) | Tag-lite (Ki nM) |
| Adrenergic | beta2 | Isoproterenol (1) - Agonist | 387 | 425 |
| Alprenolol (2) - Antagonist | 1 | 0.40 | ||
| ICI 118551 (2) - Antagonist | 0.31 - 0.63 | 0.33 |
(1) Sato et al. Eur J Pharmacol, (1996) 315, 363-367
(2) Baker et al. Eur J Pharmacol, (2005) 144, 317-322
For more information or To Place an Order:
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