Alpha 1A receptor ligand binding assay
Adrenergic alpha 1A binding assay starter pack
| Products | Product reference | Product insert | MSDS |
| Alpha adrenergic receptor red antagonist - 5000 tests | L0010RED | ||
| Tag-lite Adrenergic Alpha 1A (1 Million labeled cells) | C1TT1ADR1 | Contact us | - |
| Tag-lite buffer - 100 ml (5X) | LABMED | - | - |
The binding assay starter pack consists of three references, the labeled cells, the fluorescent ligand and the Tag-lite buffer. Each component must be ordered separately. The cells express the SNAP-receptor labeled with terbium cryptate (labeling performed with Tag-lite SNAP-Lumi4 Tb). The labeled cells are proposed in a 1 million cells packaging, to achieve a minimum of 100 tests at a cell density of 10,000 cells/well.
- This antagonist is a prazosin derivative labeled with a red emitting HTRF fluorescent probe
- This ligand has been successfully used in an a1A Adrenergic receptor Tag-lite binding assays
- Concentration: 100µM
- Buffer: HEPES 50mM, pH7.4 + 10%DMSO
- Molecular Weight: 1070
| Related products | Product reference | Product insert |
| Plasmid pSNAP-Alpha1A | PSNAPADR1 | |
| Tag-lite SNAP-Lumi4Tb - 2 nmoles | SSNPTBC | - |
Assay Validation:
Alpha 1A receptor ligand binding assay was performed using the starter pack reagents.
Kd was determined and signal inhibition (IC50) by antagonists was performed with L0010RED (Tag-lite alpha adrenergic receptor red agonist) 100nM. Results obtained with radioligand assay and Tag-lite platform were very similar.
| Family | Receptor | Compound (antagonist) | Radioactivity( Ki nM) | Tag-lite (Ki nM) |
| Adrenergic | alpha 1A | Tamsulosin | 0.10 (1) | 0.103 |
| Piribedil | 813 (2) | 663 |
(1) Williams et al. Br J Pharmacol, 1999
(2) Millan et al. J Pharmacol Exp Ther, 2002
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
