GPCR ligand binding with Tag-lite technology
Cisbio Bioassays offers a comprehensive platform
to develop HTRF ligand binding assays.
- pSNAP-GPCR plasmids to transiently express the GPCR of interest
- Tag-lite SNAP-Lumi4TB substrat to label the SNAP-Tag
- Tag-lite fluorescent ligands
Features:
- Single technology and cellular platform for all GPCR assays
- Unique non-radioactive ligand binding assays
- Miniaturization down to < 10µl
How to develop a ligand binding assay with Tag-lite
- pSNAP-GPCR plasmids to transiently express the GPCR of interest
- pSNAP plasmid to construct your own target
- Tag-lite SNAP-Lumi4TB substrate to label the SNAP-Tag (available in 3 pack sizes) and recommended Tag-lite labeling medium, optimized medium for SNAP- and CLIP-tags labeling.
- Tag-lite fluorescent ligands
- Validated in a ligand binding assay on the Tag-lite appropriate target
Cisbio can help develop your own GPCR dimer assay.
