GPCR Solutions
Assay any GPCR using a single technology
Gs, Gi and Gq Functional Assays
GPCRs carry information within cells via two major signaling pathways: regulation of cAMP levels and increases in intracellular Ca2+ triggered by inositol (1,4,5) tri-phosphate (IP3). These signaling pathways are activated by the specific G protein associated with the receptor. Gs and Gi coupled receptors result in variations of cAMP while Gq coupled GPCRs activate phospholipase C (PLC) and trigger the inositol phosphate (IP) cascade. Cisbio Bioassays has developed a full line of quality, ready-to-use kits capable of monitoring activation of Gs, Gi and Gq coupled receptors.
Features:
- Single technology for all GPCR assays
- Simple and rapid assay protocols
- Stable EC50 even after several day incubation
- Miniaturization down to < 10µl
- Low false positive, false negative results
Products:
Applications:
- Gs, Gi and Gq coupled receptor activation
- Agonist / antagonist screening
- Inverse agonist testing for constitutively active receptors
- Phosphodiesterase (PDE) monitoring
- Adenylate cyclase activity assessment
Related ELISAs:
