GPCR Solutions

Assay any GPCR using a single technology

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GPCR Solutions - Assay any GPCR using a single technology
Gs, Gi and Gq Functional Assays

GPCRs carry information within cells via two major signaling pathways: regulation of cAMP levels and increases in intracellular Ca2+ triggered by inositol (1,4,5) tri-phosphate (IP3). These signaling pathways are activated by the specific G protein associated with the receptor. Gs and Gi coupled receptors result in variations of cAMP while Gq coupled GPCRs activate phospholipase C (PLC) and trigger the inositol phosphate (IP) cascade. Cisbio Bioassays has developed a full line of quality, ready-to-use kits capable of monitoring activation of Gs, Gi and Gq coupled receptors.

Features:
  • Single technology for all GPCR assays
  • Simple and rapid assay protocols
  • Stable EC50 even after several day incubation
  • Miniaturization down to < 10µl
  • Low false positive, false negative results


Products:

Applications:
  • Gs, Gi and Gq coupled receptor activation
  • Agonist / antagonist screening
  • Inverse agonist testing for constitutively active receptors
  • Phosphodiesterase (PDE) monitoring
  • Adenylate cyclase activity assessment

Related ELISAs: