ApoA1 and ApoB assays
Apolipoprotein A1 (ApoA1) and Apolipoprotein B (ApoB) belong to the lipoprotein family, which plays important roles in lipid metabolism. While ApoA1 is the main component of HDL (high-density lipoprotein, or "good cholesterol"), ApoB is the main component of LDL and VLDL (low and very-low density lipoprotein, or "bad cholesterol").
The transcription of ApoA1 and ApoB genes is up-regulated by numerous transcription factors including PPAR,RXR, HNF4alpha, and LXR.
The levels of ApoA1 and ApoB are important markers for evaluating the risk of arteriosclerosis.
- Cost effective alternative to ELISA
- Amenable to automation and HTS
- Monoclonal antibody based assays
- Low cross reactivity with other Apolipoproteins
- Apolipoprotein quantification in cell supernatants or whole cells
- Evaluation of nuclear receptor activation by measuring the expression of ApoA1 and/or ApoB downstream from the DNA-binding domain.
Assay Principle:
The Apo-A1 and Apo-B assays can be run with cell supernatants or whole cells using the protocols shown here. Apolipoproteins are measured using sandwich immunoassays involving two monoclonal antibodies: anti-Apo MAb1 labeled with Eu-Cryptate and anti-Apo MAb2 labeled with d2. These antibodies may be pre-mixed and added in a single dispensing step to further streamline the protocol.
The assay can be run either under a cell-supernatant assay protocol (two-plate), or be further streamlined to a cell-based assay protocol (one-step).
Product specifications:
| Apo-A1 | Apo-B | |
| Antibody Incubation | 3h at RT | 3h at RT |
| Assay range | Cell-based: 0 -500 ng/mL (one-plate) Cell-supernatant: 0 - 4000 ng/mL (two-plate) |
Cell-based: 0 -1000 ng/mL (one-plate) Cell-supernatant 0 - 4000 ng/mL (two-plate) |
| Extended range | Cell-based: 1000 ng/mL (one-plate) Cell-supernatant: 8000 ng/mL (two-plate) |
Cell-based: 2000 ng/mL (one-plate) Cell-supernatant: 8000 ng/mL (two-plate) |
| Detection limit | Cell-based: 0.4 ng/mL (one-plate) Cell-supernatant 3.3 ng/mL (two-plate) |
Cell-based: 0.6 ng/mL (one-plate) Cell-supernatant 4.3 ng/mL (two-plate) |
| Specificity | Low cross reactivity with: ApoB-100, ApoA2, ApoC1, ApoC2, ApoC3, ApoE | Low cross reactivity with: ApoA1, ApoA2, ApoC1, ApoC2, ApoC3, ApoE |
Assay performances
| Cross-reactivity (%) | |
| ApoA1 | 100 |
| ApoB-100 | 0.044 |
| ApoA2 | 0.037 |
| ApoC1 | 0.014 |
| ApoC2 | 0.004 |
| ApoC3 | 0.005 |
| ApoE | 0.004 |
| Cross-reactivity (%) | |
| ApoB-100 | 100 |
| ApoA1 | 0.004 |
| ApoA2 | < 0.001 |
| ApoC1 | < 0.001 |
| ApoC2 | 0.001 |
| ApoC3 | 0.006 |
| ApoE | 0.084 |
Ordering Info:
| Description | Quantity | Cat no. | Product Insert |
| ApoA1 | 1,000 tests | 64APAPEB | |
| ApoA1 bulk | 2,000 tests | 64APAPEC | |
| ApoB | 1,000 tests | 64APBPEB | |
| ApoB bulk | 20,000 tests | 64APBPEC |
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
