Amyloid β1-40 HTRF® Assay
Amyloid β1-40 and β1-42 peptides are involved in the appearance of Alzheimer's disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, β and γ secretase, which cleave the amyloid precursor protein. Cisbio Bioassays developed an assay for the measurement of amyloid β1-40 peptides based on our proprietary HTRF® technology. This assay can be rapidly performed, easily miniaturized and is highly amenable to automation.
- Cell-based functional assay
- Simple and rapid assay protocol
- Monoclonal antibody-based
- Amenable to automation
- Amyloid β1-40 peptide measurement
- Indicator of secretase activity
Assay Principle:
The HTRF® Amyloid β1-40 HTRF assay is a sandwich immunoassay which uses two monoclonal antibodies which recognize different epitopes within the β1-40 peptide. One antibody is labeled with Eu3+-Cryptate and the second with XL-665. The assay can be used with whole cells or cell supernatants.
Assay Protocol:
The Amyloid β1-40 peptide assay can be performed in a single microplate, from cell stimulation through HTRF® signal detection. The quantification process involves the addition of the two specific antibodies-conjugates which can be pre-mixed allowing for a single reagent addition protocol. This simplified protocol can be miniaturized and is suitable for HTS.
Product Specifications:
| Detection limit: | 13.6 pg/ml |
| Dynamic range: | 7.4 - 1600 pg/ml |
| Sample compatibility: | Cell-based or cell supernatants |
| Specificity: | No cross-reaction with Amyloid β 1-42 and β 1-43 |
Figure 1: Amyloid β1-40 peptide standard curve was performed using the assay protocol outlined in the product insert.
Ordering Info:
Amyloid β1-40 Assay Reagents:
| Description | Quantity* | Cat no. | Product Inserts |
| Amyloid β1-40 peptide | 1,000 tests | 62B40PEB | |
| Amyloid β1-40 peptide | 20,000 tests | 62B40PEC | |
| *based on 20 µl assay volume | |||
For more information or To Place an Order:
Europe and other countries
+33(0)466 796 705
