Biomarkers
One Technology...Unlimited Applications
Cisbio Bioassays has developed a line of high quality assays including cytokine, insulin, histamine and amyloid β1-40 peptide assays to fulfil the needs of therapeutic areas such as inflammation, metabolic disease and CNS therapeutic areas. These assays are based on our patented HTRF® technology and are ideal for basic R&D as well as primary and secondary screening. If we don't have a ready-to-use kit for your assay of interest, Cisbio Bioassays has a line of toolbox reagents for flexible assay development. Our team of scientists is also available through our custom labeling and full assay development services.
- Simple and rapid assay protocols
- Reproducible, highly sensitive assays
- Flexible toolbox reagents
- Amenable to automation
- Miniaturization down to < 10µl
Biomarkers Solutions:
- Inflammation monitoring
- Insulin quantification
- Biomarker discovery
- Allergy and asthma research
- Infectious disease
More biomarkers with HTRF innovations
(Case study) A customer requested the labeling of a protein to be used in a protein-protein binding assay. When the material arrived in our facilities, we set forth to define a labeling strategy that would preserve the binding affinity of the two proteins.
In this instance, we chose to perform a d2-NHS labeling, primarily because the dye is 1kDa and would limit steric hindrance problems. The second reason for choosing this method is that NHS enables rapid and soft labeling. Due to the relatively weak protein-protein binding of the pair, the custom labeling team chose to use high protein concentrations with a high ratio of d2 for this component. This would give the customer a higher working stock with a higher percentage of labeled material to increase the chances of success with the assay. Following a quick protocol optimization, the batch protein was labeled and the remaining unbound d2 was separated via a G25 (from GE). Combined with the Europium labeled binding partner, the customer was able to use the d2 labeled material to develop a protein-protein binding assay.
