The Tag-lite® ligand binding assay is a homogeneous alternative to radioligand binding assays for HTS and compound profiling. The assay is based on the competition between the fluorescent ligand and compound.
Muscarinic M1 receptor ligand binding assay
Assay Validation
Please note that the data below should be considered only as an example. Results may vary from one HTRF compatible reader to another.
The Muscarinic M1 receptor ligand binding assay was performed using:
- cells expressing the SNAP-receptor labeled with terbium cryptate
- ligand labeled with a red-emitting HTRF fluorescent probe
Results obtained with this assay were very similar to those obtained with the radio ligand reference method.
Literature
Title
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Implementation of Cisbio’s HTRF® M1 muscarinic receptor related assays on CyBio liquid handling solutions for small and high throughput
Jean A, Tardieu JL, M. Préaudat M, Degorce F, Undisz K
Miptec 2012, Basel, Switzerland / SLAS 2013, Orlando USA
Title
Related products
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Implementation of Cisbio’s HTRF® M1 muscarinic receptor related assays on CyBio liquid handling solutions for small and high throughputJean A, Tardieu JL, M. Préaudat M, Degorce F, Undisz K
Miptec 2012, Basel, Switzerland / SLAS 2013, Orlando USA