IP-One HTRF® assay

IP-One is the unique GPCR Gq second messenger accumulation assay.

GPCR Gq stimulation is known to induce phospholipase C (PLC) activation and trigger the Inositol Phosphate (IP) cascade.

IP1, a downstream metabolite of IP3, accumulates in cells following Gq receptor activation and is stable in the presence of LiCl, making it ideal for GPCR (Gq) functional assays. Cisbio Bioassays has developed and patented a highly accurate HTRF assay for measuring IP1 in 96-, 384- and 1536-well formats, enabling an HTS-compatible assay.

Features

Cell-based functional assay
Second messenger accumulation assay
Assay formats 100 µL and 20 µL
Miniaturization down to < 10µl
Compatible with fresh or frozen cells

Applications

Gq coupled receptor activation
Assess agonists, antagonists, allosteric modulators and inverse agonists
Detection of slow acting agonist in functional assay

Related applications and services

Cell surface receptor assays

Tag-lite ligand binding assays

HTRF® functional assays

Assay Principle
Product Specifications
Assay Performances
Part#, inserts & MSDS
Literature

The IP-One assays are competitive immunoassays that use terbium cryptate-labeled anti-IP1 MAb and d2-labeled IP1. LiCl is added to the cell stimulation buffer, causing the accumulation of IP1 upon receptor activation. The assay can be run in a single microplate and requires just a single 1 hour incubation following cell stimulation.

GPCR IP1 assay protocol

Detection Limit:	5 nM
Dynamic Range:	5 - 3000 nM
S/B:	20 (depending on reader performance)
EC50:	100 nM
Z':	0.91 (20 µL, 384 wells)
Specificity:	No cross-reactivity with 50 µM Myo-inositol, PIP2, IP2, IP3, IP4 or PIP3
Sample types:	Living or frozen cells
Additive tolerance:	> 5% DMSO

IP One standard curve

IP1 standard curve performed using protocol outlined in IP-One Tb assay product insert.

The HTRF IP-One kit is a proven assay for assessing Gq coupled receptor activation. A side-by-side comparison was run between the HTRF IP-One kit and an isotopic inositol cascade method using a range of Gq coupled receptors and their associated agonist. The EC50 values calculated using HTRF and isotopic methods were comparable for all the receptors tested (table).

		EC50
GPCR target	Compound	HTRF®	Isotopic method
Muscarinic M1	Acetylcholine Carbachol	71 nM 296 nM	42 nM 300 nM
Vasopressin V1A	Vasopressin Vasopressin	1 nM 1.6 nM	0.4 nM 0.4 nM
Oxytocin OT (Gq)	Oxytocina	13 nM	7 nM
Histamine H2 (G16)	Amthamine	21 nM	16 nM
Purinergic P2Y1	2-methylthio ADP	6.8 nM	ND
Cholecystokinine CCK1	CCK8 Sulfate3	2 nM	43 nM
Chemokine CCRS (G16)	RANTES MIP1 α	76 nM 48 nM	26 nM ND
HupCar	Calcium	2.9 nM	ND
Endothelin Etb	Endothelin 2 Ala-endothelin3	82 nM 70 nM	83 nM 93 nM
TRHI (Gq)	TRH	0.8 nM	ND
GB1+GB2 (Gqi9)	GABA	980 nM	484 nM
mGluR 1	Quisqualate	75 nM	75 nM
mGluR5	Quisqualate	13 nM	9 nM
Muscarinic M3	Acetylcholine	20 µM	ND
Purynergic P2Y1	UTP ATP	2.1 µM 1.6 µM	ND ND

Ordering Info

Description	Cat. no	Product insert	MSDS
IP-One Tb kit - 1,000 tests	62IPAPEB	pdf	pdf
IP-One Tb kit - 20,000 tests	62IPAPEC	pdf	pdf
IP-One Tb kit -2 X 96 tests	62IPAPEF	pdf	pdf
IP-One Tb kit - 100,000 tests	62IPAPEJ	pdf	pdf
IP-One Tb kit - 1 M tests (10 packs 62IPAPEJ)	62IPAPEM	pdf	pdf

Companion products

Description	Cat. no	Product insert	MSDS
Lysis buffer 5 (IP-One Tb kit 1,000 tests) - 13 ml	62CL5FDD	-	-
Lysis Buffer 6 (IP-One Tb kits 20, & 100,000 tests)	62CL6FDF	-	-
IP-One calibrator	62IP1CDA	-	pdf
IP-One stimul. Buffer 5X - 8 mL	62IP1FDC	-	pdf
IP-One stimul. Buffer 5X - 100 mL	62IP1FDG	-	pdf
IP-One Tb control	62IPATDA	-	pdf
96 half-well white plate cell culture - 8 plates	66PL8ZZZ	-	-

View all
Scientific paper21
Poster14
Application note8
Flyer2
Presentation11

View all

Title

Related products

Download

Illuminating the activation mechanisms and allosteric properties of metabotropic glutamate receptors
Doumazane E, Scholler P, Fabre L, Zwier JM, Trinquet E, Pin JP, Rondard P.
Proc Natl Acad Sci U S A. 2013.

IP-One HTRF® assay
Tag-lite® substrates
Biased agonism of the calcium-sensing receptor
Thomsen AR, Hvidtfeldt M, Bräuner-Osborne H.
Cell Calcium. 2012;51(2):107-16.

cAMP cell-based assay
IP-One HTRF® assay
Phospho-ERK (Cellul'erk)
Rare MTNR1B variants impairing melatonin receptor 1B function contribute to type 2 diabetes
Bonnefond A, Clément N, Fawcett K, Yengo L, Vaillant E, Guillaume JL, Dechaume A, Payne F, Roussel R, Czernichow S, Hercberg S, Hadjadj S, Balkau B, Marre M, Lantieri O, Langenberg C, Bouatia-Naji N; The Meta-Analysis of Glucose and Insulin-Related Traits Consortium (MAGIC), Charpentier G, Vaxillaire M, Rocheleau G, Wareham NJ, Sladek R, McCarthy MI, Dina C, Barroso I, Jockers R, Froguel P.
Nat Genet. 2012 Jan 29. doi: 10.1038/ng.1053.
IP-One HTRF® assay
A new approach to analyze cell surface protein complexes reveals specific heterodimeric metabotropic glutamate receptors.
Doumazane E, Scholler P, Zwier JM, Eric T, Rondard P, Pin JP.
FASEB J. 2011;25(1):66-77.
IP-One HTRF® assay
Trans-activation between 7TM domains: implication in heterodimeric GABAB receptor activation.
Monnier C, Tu H, Bourrier E, Vol C, Lamarque L, Trinquet E, Pin JP, Rondard P.
EMBO J. 2011;30(1):32-42.

IP-One HTRF® assay
Tag-lite® toolbox
G protein activation by serotonin type 4 receptor dimers: evidence that turning on two protomers is more efficient
Pellissier LP, Barthet G, Gaven F, Cassier E, Trinquet E, Pin JP, Marin P, Dumuis A, Bockaert J, Banères JL, Claeysen S.
J Biol Chem. 2011 Mar 25;286(12):9985-97.

cAMP cell-based assay
IP-One HTRF® assay
Selective orthosteric free fatty acid receptor 2 (FFA2) agonists: identification of the structural and chemical requirements for selective activation of FFA2 versus FFA3
Schmidt J, Smith NJ, Christiansen E, Tikhonova IG, Grundmann M, Hudson BD, Ward RJ, Drewke C, Milligan G, Kostenis E, Ulven T
J Biol Chem. 2011;286(12):10628-40.

cAMP cell-based assay
IP-One HTRF® assay
THRX-198321 is a bifunctional muscarinic receptor antagonist and beta2-adrenoceptor agonist (MABA) that binds in a bimodal and multivalent manner
Steinfeld T, Hughes AD, Klein U, Smith JA, Mammen M.
Mol Pharmacol. 2011;79(3):389-99.

cAMP cell-based assay
IP-One HTRF® assay
Monitoring Gq-coupled receptor response through inositol phosphate quantification with the IP-One assay.
Trinquet E, Bouhelal R, Dietz M
Expert Opin. Drug Discov. (2011);6(10):981-994.
IP-One HTRF® assay
A high-throughput small-molecule ligand screen targeted to agonists and antagonists of the G-protein-coupled receptor GPR54.
Kuohung W, Burnett M, Mukhtyar D, Schuman E, Ni J, Crowley WF, Glicksman MA, Kaiser UB.
J Biomol Screen. 2010;15(5):508-17.
IP-One HTRF® assay
Development of an improved IP(1) assay for the characterization of 5-HT(2C) receptor ligands
Zhang JY, Kowal DM, Nawoschik SP, Dunlop J, Pausch MH, Peri R.
Assay Drug Dev Technol. 2010;8(1):106-13.
IP-One HTRF® assay
Subtlety of the structure-affinity and structure-efficacy relationships around a nonpeptide oxytocin receptor agonist.
Frantz MC, Rodrigo J, Boudier L, Durroux T, Mouillac B, Hibert M.
J Med Chem. 2010 Feb 25;53(4):1546-62.

cAMP cell-based assay
IP-One HTRF® assay
The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals
Martín R, Durroux T, Ciruela F, Torres M, Pin JP, Sánchez-Prieto J.
J Biol Chem. 2010;285(23):17907-17.

cAMP cell-based assay
IP-One HTRF® assay
GalR2-positive allosteric modulator exhibits anticonvulsant effects in animal models.
Lu X, Roberts E, Xia F, Sanchez-Alavez M, Liu T, Baldwin R, Wu S, Chang J, Wasterlain CG, Bartfai T
Proc Natl Acad Sci U S A. 2010;107(34):15229-34.

cAMP cell-based assay
IP-One HTRF® assay
Inhibition of heterotrimeric G protein signaling by a small molecule acting on Galpha subunit.
Ayoub MA, Damian M, Gespach C, Ferrandis E, Lavergne O, De Wever O, Baneres JL, Pin JP, Prevost GP
J Biol Chem. 2009;284(42):29136-45.

cAMP cell-based assay
IP-One HTRF® assay
A One-Day, Dispense-Only IP-One HTRF Assay for High-Throughput Screening of Gq Protein-Coupled Receptors: Towards Cells as Reagents
Bergsdorf C, Kropp-Goerkis C, Kaehler I, Ketscher L, Boemer U, Parczyk K, Bader B.
Assay Drug Dev Technol. 2008;6(1):39-53
IP-One HTRF® assay
Comparison on Functional Assays for Gq-Coupled GPCRs by Measuring Inositol Monophospate-1 and Intracellular Calcium in 1536-Well Plate Format
Liu K, Titus S, Southall N, Zhu P, Inglese J, Austin CP, Zheng W.
Curr Chem Genomics. 2008;1:70-8.
IP-One HTRF® assay
Identifying Nonselective Hits from a Homogeneous Calcium Assay Screen
Cassutt KJ, Orsini MJ, Abousleiman M, Colone D, Tang W.
J Biomol Screen. 2007;12(2):285-7.
IP-One HTRF® assay
Identifying Nonselective Hits from a Homogeneous Calcium Assay Screen
Cassutt KJ, Orsini MJ, Abousleiman M, Colone D, Tang W
J Biomol Screen. 2007;12(2):285-7.
IP-One HTRF® assay
Real-Time Analysis of Agonist-Induced Activation of Protease-Activated Receptor 1/Galpha i1 Protein Complex Measured by Bioluminescence Resonance Energy Transfer in Living Cells
Ayoub MA, Maurel D, Binet V, Fink M, Prézeau L, Ansanay H, Pin JP.
Mol Pharmacol. 2007;71(5):1329-40

cAMP cell-based assay
IP-One HTRF® assay
D-myo-Inositol 1-phosphate as a surrogate of D-myo-inositol 1,4,5-tris phosphate to monitor G protein-coupled receptor activation
Trinquet E, Fink M, Bazin H, Grillet F, Maurin F, Bourrier E, Ansanay H, Leroy C, Michaud A, Durroux T, Maurel D, Malhaire F, Goudet C, Pin JP, Naval M, Hernout O, ChrÃƒÂ©tien F, Chapleur Y, Mathis G.
Anal Biochem. 2006;358(1):126-35.
IP-One HTRF® assay
Characterisation of Inositol Phosphate & cAMP Signalling from GPCRs using Homogeneous Time-Resolved Fluorescence Technology
Johnstone E KM, Armstrong S, Akli Ayoub M, Pfleger K DG
5th HTRF Symposium, Avignon, France

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HTRF® technology on the SpectraMax® i3 Multi-Mode Platform
Monnet L, Olsen C, Lebreton ML
5th HTRF Symposium, Avignon, France

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Human & Mouse TNFα assays
Molecular Devices Readers
HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway
Dell EJ, Orban M, Tardieu JL, Degorce F
5th HTRF Symposium, Avignon, France

IP-One HTRF® assay
BMG LABTECH Readers
Ex vivo IP1 determination using Cisbio’s HTRF® IP-One assay in rodent brain extracts after muscarinic M1 receptor activation
Smets T, de Waepenaert K, Hens K, Vanhoof G
5th HTRF Symposium, Avignon, France
IP-One HTRF® assay
An advanced platform for receptor biology and pharmacolology investigation
Maurel D, Brabet I, Vol C, Prézeau L
5th HTRF Symposium, Avignon, France

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Phospho-ERK (Cellul'erk)
Next-Generation HTS Instrument Discovers Low Affi nity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway.
Dell EJ, Tardieu JL, Degorce F, Fejtl M
SLAS 2012, San Diego, California, USA

IP-One HTRF® assay
HTRF® compatible readers
Implementation of Cisbio’s HTRF® M1 muscarinic receptor related assays on CyBio liquid handling solutions for small and high throughput
Jean A, Tardieu JL, M. Préaudat M, Degorce F, Undisz K
Miptec 2012, Basel, Switzerland / SLAS 2013, Orlando USA/5th HTRF Symposium, Avignon, France.

Muscarinic M1 receptor ligand binding assay
IP-One HTRF® assay
New cell-based assays for 5-HT receptor investigations using Tag-lite technology
Dupuis E, Blanc E, Roux T, Lamarque L, Tinel N, Gasparaik V, Wagner P, Bonnet D, Bourguignon JJ, Hounsou C, Oueslati N, Belhocine A, Rodriguez T

IP-One HTRF® assay
Tag-lite® plasmids
Homogeneous High Throughput Live Cell GPCR Functional and Surface Binding Assays
Sirenko O, N'Garwate E, Tardieu JL, Lebreton ML, Jaga D, Katzlinger M, Monnet L, Cardonnel C, Olsen C, Degorce F, Cromwell EF"

cAMP cell-based assay
IP-One HTRF® assay
HTRF® compatible readers
Temporal expression analysis of endogenous GPCRs in four different mouse primary neuronal cultures
Roubert C, Bas M, Guenneau S , Jung M, Oury-Donat F, Didier M
40th SFN Anunual Meeting, San Diego, USA
IP-One HTRF® assay
High throughput hit profiling with the HTRF® IP-One Terbium kit to determine agonists, inverse agonists and antagonists
Dietz M, Lacoste C, Christensen K, Danner MC, Goepfert C, Schafer R, Schirmer V, Graf M, Enderle T
Miptec 2009, Basel, Switzerland
IP-One HTRF® assay
Identification of human endogenous calcitonin receptor in 293H cell line : characterization by cyclic AMP measurement
Sabatier R, Wang SR, Piwinca D, Jolas T, Fouchaq B
SBS 13th annual conference. April 2007, Montreal, Canada

IP-One HTRF® assay
cAMP cell-based assay
IP-One, a HTRF® homogeneous assay to monitor functional activation of Gq coupled receptors in a HTS format
Mathis M, Hammerl I, Martinez S, Amoravain M, Bouhelal R
SBS 12th Annual Conference. September 2006, Seattle, USA
IP-One HTRF® assay
Evaluation of IP-One, a new HTRF® assay to monitor Gq coupled GPCR response. Comparison under HTS conditions with FLIPR
Tozawa-Takahashi F, Tanaka Y, Nishida K, Inagaki S, Tardieu JL, Sulocha S
SBS 11th Annual Conference. September 2005, Geneva, Switzerland
IP-One HTRF® assay
HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway
An application Note from BMG LABTECH

IP-One HTRF® assay
HTRF® compatible readers
HTRF® cAMP Dynamic 2 and IP-One Assays Using the SpectraMax M5e Multi-Detection Microplate Reader
An Application Note from Molecular Devices corporation

HTRF® compatible readers
cAMP cell-based assay
IP-One HTRF® assay
Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH
An application note from BMG LABTECH

IP-One HTRF® assay
HTRF® compatible readers
HTRF®: IP-One Terbium-based assay performed on BMG LABTECH's PHERAstar Plus
An application Note from BMG LABTECH

HTRF® compatible readers
IP-One HTRF® assay
cAMP and IP-One HTRF assays on the new SpectraMax M5e multi-detection
An Application Note from Molecular Devices corporation

HTRF® compatible readers
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IP-One HTRF® assay
IP-One assay: the new solution for GPCR investigation

IP-One HTRF® assay
HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers
An application Note from BMG LABTECH

HTRF® compatible readers
IP-One HTRF® assay
cAMP/IP-One HTplex cell-based experiment performed on Mithras LB 940 using HTRF® technology
An Application Note from Berthold technologies

HTRF® compatible readers
cAMP cell-based assay
IP-One HTRF® assay
HTRF® GPCRs: Kits and reagents for ligands binding and functional assays

Phospho-ERK (Cellul'erk)
Phospho-AKT (Ser473) & Phospho-AKT (Thr308)
cAMP cell-based assay
IP-One HTRF® assay
IP-One : A stable second messenger of PLC-coupled receptors.

IP-One ELISA assay
IP-One HTRF® assay
Ultra HTS at Bayer: use of IP-One and Tag-lite® assays in GPCR drug discovery
Katrin Nowak-Reppel (Bayer Healthcare)
5th HTRF Symposium, Avignon, France

IP-One HTRF® assay
Tag-lite® binding assays
Experience on HTRF® technology in public-private collaborations
Jose Manuel Brea Floriani (USEF Platform, University of Santiago de Compostela)
5th HTRF Symposium, Avignon, France

IP-One HTRF® assay
HTRF® Services
Structure, function and pharmacology of G protein-coupled receptors: State-of-the-art and future challenges
Hans Bräuner Osborne (University of Copenhagen)
5th HTRF Symposium, Avignon, France

cAMP cell-based assay
IP-One HTRF® assay
Phospho-ERK (Cellul'erk)
Identification and characterization of allosteric modulators of GPCRs: The utility of HTRF and incorporation into generalised screening strategies
Jerman J
ELRIG 2012, Cisbio Workshop.

cAMP cell-based assay
IP-One HTRF® assay
HTRF assays of IP1, cAMP and pERK pathways employed to study biased signaling of the calcium-sensing receptor
Bräuner-Osborne H
ELRIG 2012, Cisbio Workshop.

cAMP cell-based assay
IP-One HTRF® assay
Phospho-ERK (Cellul'erk)
Two GPCR Case Studies with the IP-One Eu and Tb Assay Kits. A Positive Modulator and an Inverse Agonist Project
Martin Graf, F
SBS 14th annual conference. April 2008, St Louis, USA
IP-One HTRF® assay
qHTS for Identifying the Cell Membrane Permeable Inositol Monophosphatase Inhibitors with HTRF® IP-One Reagents
Zheng W
HTRF® 3rd Annual Symposium 2007, Sonoma, CA, USA
IP-One HTRF® assay
Novel Functional Assay Approaches for GPCR Ligand Discovery. Comparison of three different technologies to assess slow acting agonist activity. FLIPR™, CellKey™ and IP-One HTRF®
Bouhelal R
Screening Europe 2007, Barcelona, Spain

IP-One HTRF® assay
cAMP cell-based assay
Case Study: HTS of GPR-54 with IP-One Assay
Glicksman MA
Assays and Cellular Targets 2006

IP-One ELISA assay
IP-One HTRF® assay
IP-One & cAMP HTRF® Functional Cell-based Screening
Bader B, Klotz M, Zopf D, Glienke J, Martinez S
IIR Conference 2006, London, United kingdom
IP-One HTRF® assay
A new HTRF® inositol phosphate assay to monitor Gq coupled GPCR responses. A functional assay to monitor the activation of Gq coupled receptors in a HTS format.
Bouhelal R
5th annual conference of the Society for Biomolecular Screening. September 1999, Edinburgh
IP-One HTRF® assay

Scientific paper21

Title

Related products

Download

Illuminating the activation mechanisms and allosteric properties of metabotropic glutamate receptors
Doumazane E, Scholler P, Fabre L, Zwier JM, Trinquet E, Pin JP, Rondard P.
Proc Natl Acad Sci U S A. 2013.

IP-One HTRF® assay
Tag-lite® substrates
Biased agonism of the calcium-sensing receptor
Thomsen AR, Hvidtfeldt M, Bräuner-Osborne H.
Cell Calcium. 2012;51(2):107-16.

cAMP cell-based assay
IP-One HTRF® assay
Phospho-ERK (Cellul'erk)
Rare MTNR1B variants impairing melatonin receptor 1B function contribute to type 2 diabetes
Bonnefond A, Clément N, Fawcett K, Yengo L, Vaillant E, Guillaume JL, Dechaume A, Payne F, Roussel R, Czernichow S, Hercberg S, Hadjadj S, Balkau B, Marre M, Lantieri O, Langenberg C, Bouatia-Naji N; The Meta-Analysis of Glucose and Insulin-Related Traits Consortium (MAGIC), Charpentier G, Vaxillaire M, Rocheleau G, Wareham NJ, Sladek R, McCarthy MI, Dina C, Barroso I, Jockers R, Froguel P.
Nat Genet. 2012 Jan 29. doi: 10.1038/ng.1053.
IP-One HTRF® assay
A new approach to analyze cell surface protein complexes reveals specific heterodimeric metabotropic glutamate receptors.
Doumazane E, Scholler P, Zwier JM, Eric T, Rondard P, Pin JP.
FASEB J. 2011;25(1):66-77.
IP-One HTRF® assay
Trans-activation between 7TM domains: implication in heterodimeric GABAB receptor activation.
Monnier C, Tu H, Bourrier E, Vol C, Lamarque L, Trinquet E, Pin JP, Rondard P.
EMBO J. 2011;30(1):32-42.

IP-One HTRF® assay
Tag-lite® toolbox
G protein activation by serotonin type 4 receptor dimers: evidence that turning on two protomers is more efficient
Pellissier LP, Barthet G, Gaven F, Cassier E, Trinquet E, Pin JP, Marin P, Dumuis A, Bockaert J, Banères JL, Claeysen S.
J Biol Chem. 2011 Mar 25;286(12):9985-97.

cAMP cell-based assay
IP-One HTRF® assay
Selective orthosteric free fatty acid receptor 2 (FFA2) agonists: identification of the structural and chemical requirements for selective activation of FFA2 versus FFA3
Schmidt J, Smith NJ, Christiansen E, Tikhonova IG, Grundmann M, Hudson BD, Ward RJ, Drewke C, Milligan G, Kostenis E, Ulven T
J Biol Chem. 2011;286(12):10628-40.

cAMP cell-based assay
IP-One HTRF® assay
THRX-198321 is a bifunctional muscarinic receptor antagonist and beta2-adrenoceptor agonist (MABA) that binds in a bimodal and multivalent manner
Steinfeld T, Hughes AD, Klein U, Smith JA, Mammen M.
Mol Pharmacol. 2011;79(3):389-99.

cAMP cell-based assay
IP-One HTRF® assay
Monitoring Gq-coupled receptor response through inositol phosphate quantification with the IP-One assay.
Trinquet E, Bouhelal R, Dietz M
Expert Opin. Drug Discov. (2011);6(10):981-994.
IP-One HTRF® assay
A high-throughput small-molecule ligand screen targeted to agonists and antagonists of the G-protein-coupled receptor GPR54.
Kuohung W, Burnett M, Mukhtyar D, Schuman E, Ni J, Crowley WF, Glicksman MA, Kaiser UB.
J Biomol Screen. 2010;15(5):508-17.
IP-One HTRF® assay
Development of an improved IP(1) assay for the characterization of 5-HT(2C) receptor ligands
Zhang JY, Kowal DM, Nawoschik SP, Dunlop J, Pausch MH, Peri R.
Assay Drug Dev Technol. 2010;8(1):106-13.
IP-One HTRF® assay
Subtlety of the structure-affinity and structure-efficacy relationships around a nonpeptide oxytocin receptor agonist.
Frantz MC, Rodrigo J, Boudier L, Durroux T, Mouillac B, Hibert M.
J Med Chem. 2010 Feb 25;53(4):1546-62.

cAMP cell-based assay
IP-One HTRF® assay
The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals
Martín R, Durroux T, Ciruela F, Torres M, Pin JP, Sánchez-Prieto J.
J Biol Chem. 2010;285(23):17907-17.

cAMP cell-based assay
IP-One HTRF® assay
GalR2-positive allosteric modulator exhibits anticonvulsant effects in animal models.
Lu X, Roberts E, Xia F, Sanchez-Alavez M, Liu T, Baldwin R, Wu S, Chang J, Wasterlain CG, Bartfai T
Proc Natl Acad Sci U S A. 2010;107(34):15229-34.

cAMP cell-based assay
IP-One HTRF® assay
Inhibition of heterotrimeric G protein signaling by a small molecule acting on Galpha subunit.
Ayoub MA, Damian M, Gespach C, Ferrandis E, Lavergne O, De Wever O, Baneres JL, Pin JP, Prevost GP
J Biol Chem. 2009;284(42):29136-45.

cAMP cell-based assay
IP-One HTRF® assay
A One-Day, Dispense-Only IP-One HTRF Assay for High-Throughput Screening of Gq Protein-Coupled Receptors: Towards Cells as Reagents
Bergsdorf C, Kropp-Goerkis C, Kaehler I, Ketscher L, Boemer U, Parczyk K, Bader B.
Assay Drug Dev Technol. 2008;6(1):39-53
IP-One HTRF® assay
Comparison on Functional Assays for Gq-Coupled GPCRs by Measuring Inositol Monophospate-1 and Intracellular Calcium in 1536-Well Plate Format
Liu K, Titus S, Southall N, Zhu P, Inglese J, Austin CP, Zheng W.
Curr Chem Genomics. 2008;1:70-8.
IP-One HTRF® assay
Identifying Nonselective Hits from a Homogeneous Calcium Assay Screen
Cassutt KJ, Orsini MJ, Abousleiman M, Colone D, Tang W.
J Biomol Screen. 2007;12(2):285-7.
IP-One HTRF® assay
Identifying Nonselective Hits from a Homogeneous Calcium Assay Screen
Cassutt KJ, Orsini MJ, Abousleiman M, Colone D, Tang W
J Biomol Screen. 2007;12(2):285-7.
IP-One HTRF® assay
Real-Time Analysis of Agonist-Induced Activation of Protease-Activated Receptor 1/Galpha i1 Protein Complex Measured by Bioluminescence Resonance Energy Transfer in Living Cells
Ayoub MA, Maurel D, Binet V, Fink M, Prézeau L, Ansanay H, Pin JP.
Mol Pharmacol. 2007;71(5):1329-40

cAMP cell-based assay
IP-One HTRF® assay
D-myo-Inositol 1-phosphate as a surrogate of D-myo-inositol 1,4,5-tris phosphate to monitor G protein-coupled receptor activation
Trinquet E, Fink M, Bazin H, Grillet F, Maurin F, Bourrier E, Ansanay H, Leroy C, Michaud A, Durroux T, Maurel D, Malhaire F, Goudet C, Pin JP, Naval M, Hernout O, ChrÃƒÂ©tien F, Chapleur Y, Mathis G.
Anal Biochem. 2006;358(1):126-35.
IP-One HTRF® assay

Poster14

Title

Related products

Download

Characterisation of Inositol Phosphate & cAMP Signalling from GPCRs using Homogeneous Time-Resolved Fluorescence Technology
Johnstone E KM, Armstrong S, Akli Ayoub M, Pfleger K DG
5th HTRF Symposium, Avignon, France

cAMP cell-based assay
IP-One HTRF® assay
HTRF® technology on the SpectraMax® i3 Multi-Mode Platform
Monnet L, Olsen C, Lebreton ML
5th HTRF Symposium, Avignon, France

IP-One HTRF® assay
cAMP cell-based assay
Human & Mouse TNFα assays
Molecular Devices Readers
HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway
Dell EJ, Orban M, Tardieu JL, Degorce F
5th HTRF Symposium, Avignon, France

IP-One HTRF® assay
BMG LABTECH Readers
Ex vivo IP1 determination using Cisbio’s HTRF® IP-One assay in rodent brain extracts after muscarinic M1 receptor activation
Smets T, de Waepenaert K, Hens K, Vanhoof G
5th HTRF Symposium, Avignon, France
IP-One HTRF® assay
An advanced platform for receptor biology and pharmacolology investigation
Maurel D, Brabet I, Vol C, Prézeau L
5th HTRF Symposium, Avignon, France

IP-One HTRF® assay
cAMP cell-based assay
Phospho-ERK (Cellul'erk)
Next-Generation HTS Instrument Discovers Low Affi nity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway.
Dell EJ, Tardieu JL, Degorce F, Fejtl M
SLAS 2012, San Diego, California, USA

IP-One HTRF® assay
HTRF® compatible readers
Implementation of Cisbio’s HTRF® M1 muscarinic receptor related assays on CyBio liquid handling solutions for small and high throughput
Jean A, Tardieu JL, M. Préaudat M, Degorce F, Undisz K
Miptec 2012, Basel, Switzerland / SLAS 2013, Orlando USA/5th HTRF Symposium, Avignon, France.

Muscarinic M1 receptor ligand binding assay
IP-One HTRF® assay
New cell-based assays for 5-HT receptor investigations using Tag-lite technology
Dupuis E, Blanc E, Roux T, Lamarque L, Tinel N, Gasparaik V, Wagner P, Bonnet D, Bourguignon JJ, Hounsou C, Oueslati N, Belhocine A, Rodriguez T

IP-One HTRF® assay
Tag-lite® plasmids
Homogeneous High Throughput Live Cell GPCR Functional and Surface Binding Assays
Sirenko O, N'Garwate E, Tardieu JL, Lebreton ML, Jaga D, Katzlinger M, Monnet L, Cardonnel C, Olsen C, Degorce F, Cromwell EF"

cAMP cell-based assay
IP-One HTRF® assay
HTRF® compatible readers
Temporal expression analysis of endogenous GPCRs in four different mouse primary neuronal cultures
Roubert C, Bas M, Guenneau S , Jung M, Oury-Donat F, Didier M
40th SFN Anunual Meeting, San Diego, USA
IP-One HTRF® assay
High throughput hit profiling with the HTRF® IP-One Terbium kit to determine agonists, inverse agonists and antagonists
Dietz M, Lacoste C, Christensen K, Danner MC, Goepfert C, Schafer R, Schirmer V, Graf M, Enderle T
Miptec 2009, Basel, Switzerland
IP-One HTRF® assay
Identification of human endogenous calcitonin receptor in 293H cell line : characterization by cyclic AMP measurement
Sabatier R, Wang SR, Piwinca D, Jolas T, Fouchaq B
SBS 13th annual conference. April 2007, Montreal, Canada

IP-One HTRF® assay
cAMP cell-based assay
IP-One, a HTRF® homogeneous assay to monitor functional activation of Gq coupled receptors in a HTS format
Mathis M, Hammerl I, Martinez S, Amoravain M, Bouhelal R
SBS 12th Annual Conference. September 2006, Seattle, USA
IP-One HTRF® assay
Evaluation of IP-One, a new HTRF® assay to monitor Gq coupled GPCR response. Comparison under HTS conditions with FLIPR
Tozawa-Takahashi F, Tanaka Y, Nishida K, Inagaki S, Tardieu JL, Sulocha S
SBS 11th Annual Conference. September 2005, Geneva, Switzerland
IP-One HTRF® assay

Application note8

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HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway
An application Note from BMG LABTECH

IP-One HTRF® assay
HTRF® compatible readers
HTRF® cAMP Dynamic 2 and IP-One Assays Using the SpectraMax M5e Multi-Detection Microplate Reader
An Application Note from Molecular Devices corporation

HTRF® compatible readers
cAMP cell-based assay
IP-One HTRF® assay
Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH
An application note from BMG LABTECH

IP-One HTRF® assay
HTRF® compatible readers
HTRF®: IP-One Terbium-based assay performed on BMG LABTECH's PHERAstar Plus
An application Note from BMG LABTECH

HTRF® compatible readers
IP-One HTRF® assay
cAMP and IP-One HTRF assays on the new SpectraMax M5e multi-detection
An Application Note from Molecular Devices corporation

HTRF® compatible readers
cAMP cell-based assay
IP-One HTRF® assay
IP-One assay: the new solution for GPCR investigation

IP-One HTRF® assay
HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers
An application Note from BMG LABTECH

HTRF® compatible readers
IP-One HTRF® assay
cAMP/IP-One HTplex cell-based experiment performed on Mithras LB 940 using HTRF® technology
An Application Note from Berthold technologies

HTRF® compatible readers
cAMP cell-based assay
IP-One HTRF® assay

Flyer2

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HTRF® GPCRs: Kits and reagents for ligands binding and functional assays

Phospho-ERK (Cellul'erk)
Phospho-AKT (Ser473) & Phospho-AKT (Thr308)
cAMP cell-based assay
IP-One HTRF® assay
IP-One : A stable second messenger of PLC-coupled receptors.

IP-One ELISA assay
IP-One HTRF® assay

Presentation11

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Ultra HTS at Bayer: use of IP-One and Tag-lite® assays in GPCR drug discovery
Katrin Nowak-Reppel (Bayer Healthcare)
5th HTRF Symposium, Avignon, France

IP-One HTRF® assay
Tag-lite® binding assays
Experience on HTRF® technology in public-private collaborations
Jose Manuel Brea Floriani (USEF Platform, University of Santiago de Compostela)
5th HTRF Symposium, Avignon, France

IP-One HTRF® assay
HTRF® Services
Structure, function and pharmacology of G protein-coupled receptors: State-of-the-art and future challenges
Hans Bräuner Osborne (University of Copenhagen)
5th HTRF Symposium, Avignon, France

cAMP cell-based assay
IP-One HTRF® assay
Phospho-ERK (Cellul'erk)
Identification and characterization of allosteric modulators of GPCRs: The utility of HTRF and incorporation into generalised screening strategies
Jerman J
ELRIG 2012, Cisbio Workshop.

cAMP cell-based assay
IP-One HTRF® assay
HTRF assays of IP1, cAMP and pERK pathways employed to study biased signaling of the calcium-sensing receptor
Bräuner-Osborne H
ELRIG 2012, Cisbio Workshop.

cAMP cell-based assay
IP-One HTRF® assay
Phospho-ERK (Cellul'erk)
Two GPCR Case Studies with the IP-One Eu and Tb Assay Kits. A Positive Modulator and an Inverse Agonist Project
Martin Graf, F
SBS 14th annual conference. April 2008, St Louis, USA
IP-One HTRF® assay
qHTS for Identifying the Cell Membrane Permeable Inositol Monophosphatase Inhibitors with HTRF® IP-One Reagents
Zheng W
HTRF® 3rd Annual Symposium 2007, Sonoma, CA, USA
IP-One HTRF® assay
Novel Functional Assay Approaches for GPCR Ligand Discovery. Comparison of three different technologies to assess slow acting agonist activity. FLIPR™, CellKey™ and IP-One HTRF®
Bouhelal R
Screening Europe 2007, Barcelona, Spain

IP-One HTRF® assay
cAMP cell-based assay
Case Study: HTS of GPR-54 with IP-One Assay
Glicksman MA
Assays and Cellular Targets 2006

IP-One ELISA assay
IP-One HTRF® assay
IP-One & cAMP HTRF® Functional Cell-based Screening
Bader B, Klotz M, Zopf D, Glienke J, Martinez S
IIR Conference 2006, London, United kingdom
IP-One HTRF® assay
A new HTRF® inositol phosphate assay to monitor Gq coupled GPCR responses. A functional assay to monitor the activation of Gq coupled receptors in a HTS format.
Bouhelal R
5th annual conference of the Society for Biomolecular Screening. September 1999, Edinburgh
IP-One HTRF® assay

IP-One HTRF® assay

IP-One is the unique GPCR Gq second messenger accumulation assay.

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