Disturbances in cytokine regulation are known to lead to inappropriate or ineffective immune responses. Cytokines are of particular interest in the fields of oncology, infectious diseases, allergies and autoimmune diseases. Cisbio Bioassays has developed a line of reagents for quantifying a variety of cytokines, based on our patented HTRF® technology. These reagents produce quality results, are highly sensitive and can be used with cell supernatants or whole cells.
All cytokine assays can be run with cell supernatants or whole cells using the protocols shown here. Cytokines are measured using sandwich immunoassays involving two monoclonal antibodies: anti-IL17 (MAb1) labeled with Eu-Cryptate and anti-IL17 (MAb2) labeled with d2. These antibodies may be pre-mixed and added in a single dispensing step, to further streamline the protocol. In addition, we have adapted our assay protocols to incubate the labeled antibody pair with cells during the stimulation step, and thus eliminate the need to transfer cell supernatants prior to detection. The addition of potassium fluoride stops cell stimulation and allows the HTRF signal to be read immediately.
| Incubation | 2 h at RT |
|---|---|
| Calibration range: | 0-2,000 pg/mL |
| Detection limit | 15 pg/mL NIBSC 89/520 |
| Extended range | Up to 5,000 pg/mL |
| Description | Cat. no | Product insert | MSDS |
|---|---|---|---|
| Human IL8 kit - 1,000 tests | 62IL8PEB | ||
| Human IL8 kit - 20,000 tests | 62IL8PEC |
| Description | Cat. no | Product insert | MSDS |
|---|---|---|---|
| Diluent 1 - 20 ml | 62DL1DDD | - | |
| IL8 calibrator | 62IL8CDA | - | |
| Reconstitution buffer 2 - 13 ml | 62RB2RDD | - | - |
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