Human IL2 assay

Disturbances in cytokine regulation are known to lead to inappropriate or ineffective immune responses. Cytokines are of particular interest in the fields of oncology, infectious diseases, allergies and autoimmune diseases. Cisbio Bioassays has developed a line of reagents for quantifying a variety of cytokines, based on our patented HTRF® technology. These reagents produce quality results, are highly sensitive and can be used with cell supernatants or whole cells.

Features

Cost-effective alternative to ELISA
Compatible with cell supernatants and whole cells
Streamlined single-plate protocols for HTS
Monoclonal antibody-based for increased reproducibility
Highly sensitive (< 15 pg/mL)
Small sample size needed (down to 5µL)

Applications

Cytokine quantification
Infectious disease
Allergy studies
Autoimmune disorders

All cytokine assays can be run with cell supernatants or whole cells using the protocols shown here. Cytokines are measured using sandwich immunoassays involving two monoclonal antibodies: anti-IL2 (MAb1) labeled with Eu-Cryptate and anti-IL2 (MAb2) labeled with d2. These antibodies may be pre-mixed and added in a single dispensing step, to further streamline the protocol. In addition, we have adapted our assay protocols to incubate the labeled antibody pair with cells during the stimulation step, and thus eliminate the need to transfer cell supernatants prior to detection. The addition of potassium fluoride stops cell stimulation and allows the HTRF signal to be read immediately.