HTRF® Dye labeling kits

Each HTRF dye labeling kit includes all the materials necessary for rapid cryptate or d2 dye labeling of peptides, proteins, oligonucleotides, or small molecules containing amino-groups (N-terminus or lysine residues).

The labeling procedure requires only a few steps and is performed under very mild conditions. As shown here, the process comprises an incubation step with the dessicated dye, followed by purification on a disposable column. Both the purification column and the buffer for purifying the conjugate are included in the kit, as well as a calibrator to define the ideal working dilution of the labeled material in HTRF assays (for cryptates only). Depending on the molecular weight of the target molecule and the nature of the dye, each kit enables the labeling of 5-25 μg (protein-MW 15 kDa) to 50-250 μg (antibody-MW 150 kDa).