A wide range of anti-tag HTRF fluorophore conjugates for molecular interaction study
Tagged biomolecules are a key element in studying molecular interactions and offer researchers flexibility in assay design. The most frequently used tags for developing assays consist of proteinic or peptidic structures such as GST, 6HIS, c-myc, FLAG® and HA, maltose binding protein (MBP) or small organic motifs like dinitrophenyl (DNP). HTRF has also been successfully applied to NEB's SNAP-tag® technology.
The Lumi4®-Terbium cryptate conjugates bring an additional level of flexibility, as they can be combined either with the HTRF tracers, XL665 or d2, or with other fluorophores like fluorescein or green fluorescent protein (GFP). What's more, Lumi4-Terbium cryptate reagents enable KF free assays and are fully compatible with HTRF certified readers.