The Tag-lite® ligand binding assay is a homogeneous alternative to radioligand binding assays for HTS and compound profiling. The assay is based on the competition between the fluorescent ligand and compound.
Glucagon GLP1 receptor ligand binding assay
Assay Validation
Please note that the data below should be considered only as an example. Results may vary from one HTRF compatible reader to another.
The Glucagon GLP1 receptor ligand binding assay was performed using:
- cells expressing the SNAP-receptor labeled with terbium cryptate
- ligand labeled with a red-emitting HTRF fluorescent probe
Results obtained with this assay were very similar to those obtained with the radio ligand reference method.
Literature
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Title
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Rapid Screening of a Cell-based Assay for GLP-1 Receptor Using a Natural Product Library
Larson B, Pierre N, Graham S, Tardieu JL, Degorce F, Banks P
Miptec 2012, Basel, Switzerland / SLAS 2013, Orlando USA
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A cell-based assay for GLP1 receptor ligand binding HTS using the Tag-lite technology
Bdioui S, Roux T, Costy N, Douzon S, Lamarque L, Latzko F, Trinquet E
SBS 17th Annual Conference. April 2011, Orlando, USA -
Measurement of Glucagon–like peptide 1 with the HTRF® assay
Tokuda C, Adachi K, Preaudat M
SBS 17th Annual Conference. April 2011, Orlando, USA
Poster3
Title
Related products
Download
-
Rapid Screening of a Cell-based Assay for GLP-1 Receptor Using a Natural Product Library
Larson B, Pierre N, Graham S, Tardieu JL, Degorce F, Banks P
Miptec 2012, Basel, Switzerland / SLAS 2013, Orlando USA -
A cell-based assay for GLP1 receptor ligand binding HTS using the Tag-lite technology
Bdioui S, Roux T, Costy N, Douzon S, Lamarque L, Latzko F, Trinquet E
SBS 17th Annual Conference. April 2011, Orlando, USA -
Measurement of Glucagon–like peptide 1 with the HTRF® assay
Tokuda C, Adachi K, Preaudat M
SBS 17th Annual Conference. April 2011, Orlando, USA