Cisbio Bioassays

GBCR Application Center
Skip to content

cAMP

Cisbio Bioassays offers a line of three ready-to-use assays for measuring cAMP using HTRF® technology. Each kit is optimized for detecting a specific range of cAMP concentrations to allow fine tuning of assay sensitivity. These kits enable high quality, cell-based assay screening in 96-, 384- or 1536-well formats.

cAMP Overview
Single-plate Protocol
EC50 stable from 1 hr to 7 days CHO-K1 cells were stimulated with Methylhistamine in the presence of 30 µM forskolin (EC80). 20 µl assays were performed in 384-well plates. Plates were read at times ranging from 1 hour to 7 days.
3 kits for optimized S/B & sensitivity Cisbio Bioassays offers 3 cAMP kits (femto 2, dynamic 2, HiRange) each having specific cAMP sensitivity levels and S/B. This allows researchers to choose a kit that best meets their scientific needs. Each of the 3 kits is available in 1000, 20,000 and 100,000 test sizes.