Cisbio Bioassays offers a line of three ready-to-use assays for measuring cAMP using HTRF® technology. Each kit is optimized for the detection of a specific range of cAMP concentrations, to enable assay sensitivity fine tuning. These kits give high quality, cell-based assay screening in 96-, 384- or 1536-well formats.
cAMP kits are based on a competitive immunoassay using cryptate-labeled anti-cAMP and d2-labeled cAMP. The dye d2 is our newest HTRF® acceptor fluorophore.
cAMP femto kits are the most sensitive cAMP cell-based assays. With the introduction of Lumi4®-Tb cryptate, cAMP femto is now available with two cryptates (europium or terbium). Both kits enable the detection of as little as 28 pM cAMP (0.6 fmoles/well in 20µl volume).
cAMP dynamic 2 covers a wide range of cAMP concentrations. This kit is ideal for a broad range of Gi/s assays.
With an extended signal-to-background ratio and Z' > 0.9, cAMP HiRange is streamlined for HTS. The antibody does not cross-react with ATP. membrane preparations
| Cryptate | S/B | IC20 nM* | IC50 nM* | IC80 nM* | |
|---|---|---|---|---|---|
| cAMP femto Kit | Eu3+ | >8 | 0.33 | 1.76 | 9.4 |
| cAMP femto Tb Kit | Tb2+ | >8 | 0.33 | 1.76 | 9.4 |
| cAMP dynamic 2 Kit | Eu3+ | >14 | 0.91 | 4.07 | 18.1 |
| cAMP HiRange Kit | Eu3+ | >28 | 4.27 | 22.8 | 1.21 |
* final cAMP concentration
| Description | Cat. no | Product insert | MSDS |
|---|---|---|---|
| cAMP dynamic 2 kit - 1,000 tests | 62AM4PEB | ||
| cAMP dynamic 2 kit - 20,000 tests | 62AM4PEC | ||
| cAMP dynamic 2 kit -2 X 96 tests | 62AM4PEF | ||
| cAMP dynamic 2 kit - 100,000 tests | 62AM4PEJ | ||
| cAMP dynamic 2 kit -1 M tests (10 packs 62AM4PEJ) | 62AM4PEM | ||
| cAMP femto 2 kit - 1,000 tests | 62AM5PEB | ||
| cAMP femto 2 kit - 20,000 tests | 62AM5PEC | ||
| cAMP femto 2 kit - 100,000 tests | 62AM5PEJ | ||
| cAMP HiRange kit - 1,000 tests | 62AM6PEB | ||
| cAMP HiRange kit - 20,000 tests | 62AM6PEC | ||
| cAMP HiRange kit - 100,000 tests | 62AM6PEJ | ||
| cAMP HiRange kit - 1 M tests (10 packs 62AM6PEJ) | 62AM6PEM | ||
| cAMP femto Tb kit - 1,000 tests | 62AM7PEB | ||
| cAMP femto Tb kit - 20,000 tests | 62AM7PEC | ||
| cAMP femto Tb kit - 100,000 tests | 62AM7PEJ | ||
| Lysis buffer 2 (cAMP/cGMP kits 20, & 100,000 tests) | 62CL2FDF | - | - |
| Description | Cat. no | Product insert | MSDS |
|---|---|---|---|
| cAMP dynamic 2 calibrator | 62AM4CDA | - | |
| cAMP dynamic 2 control | 62AM4TDA | - | |
| cAMP femto 2 calibrator | 62AM5CDA | - | |
| cAMP femto 2 control | 62AM5TDA | - | |
| cAMP HiRange calibrator | 62AM6CDA | - | |
| cAMP HiRange control | 62AM6TDA | - | |
| Lysis buffer 1 (cAMP/cGMP kits 1,000 tests) - 13 ml | 62CL1FDD | - | - |
| Lysis buffer 2 (cAMP/cGMP kits 20, & 100,000 tests) | 62CL2FDF | - | - |
| 96 half-well white plate cell culture - 8 plates | 66PL8ZZZ | - | - |
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